ibidi Stage Top Incubation System, Universal Fit, for 1 Chamber, CO2/O2

Applications:

Live cell imaging that requires incubator conditions on the microscope, for example:

• Chemotaxis assays
• Angiogenesis assays
• Cell migration assays
• Wound healing assays
• Proliferation assays
• Cell culture under flow

High-resolution imaging applications that require extra focus stability, for example:

• TIRF
• Confocal microscopy
• DIC microscopy

Live cell imaging using the ibidi Stage Top Incubation System shows the gap closure of MCF7 cells in a wound healing assay.

System Overview

System Components

Find a detailed description of the ibidi Stage Top Incubator components including their specifications and compatibilities here:

Live Cell Imaging: Establish in Vivo-Like Conditions on the Microscope

Cells react sensitively to changes in their environment. Factors such as temperature, humidity, and CO₂/O₂ levels significantly influence the outcome of cell culture assays. It is therefore crucial to precisely maintain the optimal conditions throughout the whole live cell imaging experiment.

The ibidi Stage Top Incubation Systems are available for one slide/dish (Universal Fit) and for multiwell plates (K-Frame). They allow for the accurate and reliable control of temperature, CO₂ and O₂ concentration, and relative humidity during short-term and long-term live cell imaging assays on any inverted microscope.

If additional control of the oxygen level is needed (e.g., in hypoxia experiments), the ibidi Stage Top Incubation Systems, CO₂/O₂ are the ideal solution.

With fast and precise regulation of important environmental parameters, the ibidi Stage Top Incubators provide stable conditions for live cell imaging directly on the microscope.

Brilliant Images Without Condensation

The Heated Lid of the ibidi Heating System reliably prevents condensation, which can reduce the imaging quality during live cell microscopy.

By heating the lid to a temperature higher than the plate, a vertical temperature gradient is created. This gradient, plus the humidity control, prevent lid condensation of the cell culture dish. The required temperature of the cell environment is constantly maintained.