Torpedo siRNA Transfection Reagent is specially designed for the transfection of small RNA (siRNA & miRNA) into mammalian cells. Lipid based transfection reagents often interact with plastic surfaces and therefore affect transfection efficiency and cell viability. ibidi’s Torpedo siRNA reagent has been specifically optimized for use with ibidi’s plastic materials. This results in a reagent with very low cytotoxicity as well as high transfection efficiencies.
≥ 90% silencing efficiency for transient knockdown of mammalian gene expression
Low siRNA concentration (1 nM) sufficient
Reagent compatible with serum
Proprietary lipids in water
Up to 1500 (96-well), or up to 375 (24-well) with 1.5 ml reagent
Knockdown of EGFP Expression after Transfection with Torpedo siRNA
Cells - stably expressing EGFP - were seeded on a µ-Plate 24 well and transfected with siRNA against EGFP or non-specific, control siRNA using Torpedo siRNA. They were imaged after 24, 48, and 72 hours showing a knockdown of EGFP expression.
Reverse siRNA Transfection Protocol for µ-Plate 24 Well:
Dr. Juliane Merl, Helmholtz Zentrum München, Germany
„We used the Torpedo siRNA Transfection Reagent for the transfection of human retinal pigment epithelial cells and experienced a reproducible and efficient knock-down of our protein of interest; even with really low amounts of siRNA.“
Dr. Juliane Merl Research Unit Protein Science Helmholtz Zentrum München Germany