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Torpedo siRNA Transfection Reagent

Torpedo siRNA Transfection Reagent View larger

A reagent for transfection of mammalian cells with siRNA or miRNA; optimized for use with ibidi µ-Slides and µ-Dishes

  • High transfection efficiency
  • Superb biocompatibility with low cytotoxicity for use in live cell imaging
  • Compatible with various cell lines
  • Ready to use

Product Details

Applications:

  • Gene silencing experiments
  • RNA research, e.g., epigenetics
  • Protein interaction studies

Torpedo siRNA Transfection Reagent is specially designed for the transfection of small RNA (siRNA & miRNA) into mammalian cells. Lipid based transfection reagents often interact with plastic surfaces and therefore affect transfection efficiency and cell viability. ibidi’s Torpedo siRNA reagent has been specifically optimized for use with ibidi’s plastic materials. This results in a reagent with very low cytotoxicity as well as high transfection efficiencies.

Technical Features:

  • ≥ 90% silencing efficiency for transient knockdown of mammalian gene expression
  • Low siRNA concentration (1 nM) sufficient
  • Reagent compatible with serum

Specifications:

Composition Proprietary lipids in water
Assays Up to 1500 (96-well), or up to 375 (24-well)
with 1.5 ml reagent
Storage -20 °C

Knockdown of EGFP Expression after Transfection with Torpedo siRNA

Cells - stably expressing EGFP - were seeded on a µ-Plate 24 well and transfected with siRNA against EGFP or non-specific, control siRNA using Torpedo siRNA. They were imaged after 24, 48, and 72 hours showing a knockdown of EGFP expression.

Reverse siRNA Transfection Protocol for µ-Plate 24 Well:

Supporting Material

Movies

User Comments

Dr. Juliane Merl, Helmholtz Zentrum München, Germany

„We used the Torpedo siRNA Transfection Reagent for the transfection of human retinal pigment epithelial cells and experienced a reproducible and efficient knock-down of our protein of interest; even with really low amounts of siRNA.“

Dr. Juliane Merl
Research Unit Protein Science
Helmholtz Zentrum München
Germany

https://www.helmholtz-muenchen.de/proteinscience/index.html

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