A fusion reagent used to incorporate lipids or amphipathic molecules into the plasma membrane of living cells
Fusogenic liposomes used as a lipid carrier for the transfer into the cellular plasma membran
Superior fusion efficiency (up to 80-100%)
Fusion process completed within 1 - 10 minutes
in cooperation with
This product is no longer in stock
Lipid imaging for monitoring turnover, traffic, and functional analysis in living cells
Lipid raft, lipid microdomain, and lipid diffusivity measurements
Blocking, induction, or replacement of lipid induced/regulated signal cascades
Re-incorporation of lipids into mutant cells affected in lipid biosynthesis
After membrane fusion, cells can immediately be used for further analysis. If necessary, more than one lipid of interest can be incorporated at once. Depending on your experimental needs, the fusion process can be monitored by fluorescence microscopy.
Bodipy FL C12-galactocerebroside and TopFluor cholesterol were incorporated into the plasma membrane of CHO cells using Fuse-It-L (5 min incubation).
Principle of Membrane Fusion
The incorporation of small liposomal carriers into the plasma membrane of mammalian cells is the idea behind all of ibidi’s Fuse-It products. Liposomal carriers are able to attach and instantly fuse with plasma membranes in a physicochemical-driven manner. ibidi’s new Fuse-It reagents efficiently use this mechanism and fuse with mammalian cell surfaces immediately upon contact. Therefore, this novel technique makes the transfer of molecules independent of biological processes, such as endocytosis, pinocytosis, or specific receptor binding.