From left to right: CHO cells fused with Fuse-It green, Fuse-It red, Fuse-It dred, and Fuse-It IR for 1 min.
Principle of Membrane Fusion
The incorporation of small liposomal carriers into the plasma membrane of mammalian cells is the idea behind all of ibidi’s Fuse-It products. Liposomal carriers are able to attach and instantly fuse with plasma membranes in a physicochemical-driven manner. ibidi’s new Fuse-It reagents efficiently use this mechanism and fuse with mammalian cell surfaces immediately upon contact. Therefore, this novel technique makes the transfer of molecules independent of biological processes, such as endocytosis, pinocytosis, or specific receptor binding.
Peter Krenn, Laboratory for Immunological and Molecular Cancer Research, Salzburg, Austria
“Investigating the homing of primary human chronic lymphocytic leukemia cells in NOD-SCID mice has always been a demanding experimental approach due to the delicate cells and the high working load. Using Fuse-It-Color dyes, we were able to label the cells in an easy, quick, and robust manner, which, at the same time, did not interfere with the homing capacities of the cells, thereby making a complex experiment easier.”
Peter Krenn Laboratory for Immunological and Molecular Cancer Research Salzburg Austria
Stephane R. Gross, Aston University, Birmingham, United Kingdom
“For my group, the efficient labeling of cells is an extremely important issue. Since most of our cells are very sensitive to changing medium conditions, mild and fast labeling is a must. We have found, in the very short time we have used the system and therefore without real optimization, that both the Fuse-It-Color and Fuse-It-P were very well functional, with the latter perfectly capable of delivering our target proteins into HeLa cells. Such delivery was obtained using relatively low amounts of exogenous proteins and incubation times as short as 5 minutes.”
Dr. Stephane R. Gross Lecturer in Cellular Biology School of Life and Health Sciences Aston University Birmingham United Kingdom