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Transfection, Transduction, and Membrane Fusion

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Application Notes

AN 07: Gene Transfection (PDF)
Example showing how protocols for gene transfection can easily be adapted to the work in cell culture channels

AN 28: Adenoviral Transduction of Human Cells (PDF)
Detailed protocol for handling recombinant adenoviruses, plus designing an approach for transducing human cells

AN 41: Lentiviral Transduction of Human Cells (PDF)
Detailed protocol for handling recombinant lentiviruses, plus designing an approach for transducing human cells

AN 52: Transfer of R-Phycoerythrin into CHO-K1 cells using Fuse-It-P (PDF)
Detailed instructions for the transfer of the protein R-Phycoerythrin into CHO-K1 cells using Fuse-It-P in the µ-Dish 35 mm, high

AN 43: Transfer of mRNA into L929 Cells using Fuse-It-mRNA (PDF)
Detailed protocol for handling recombinant lentiviruses, plus designing an approach for transducing human cells

AN 29: Transfer of mRNA into iPSC-derived Cardiomyocytes Using Fuse-It-mRNA (PDF)
Detailed instructions for the optimization of the Fuse-It-mRNA protocol for iPSC-derived Cardiomyocytes in the µ-Slide VI 0.4

AN 54: Knockdown of GFP-Expression—A Comparison of Fusion and Lipofection (PDF)
Instructions on the handling of Fuse-It-siRNA, as well as a comparison of various siRNA transfection alternatives