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Tube Formation Assays in the µ-Plate Angiogenesis 96 Well
By utilizing ibidi’s μ-Plate Angiogenesis 96 Well the anti-angiogenic potential of substances can be tested with functional tube formation assays. With this fully ANSI/SLAS (SBS) compliant and robotics compatible screening plate, compounds can be tested for their influence on structural functionality, using an in vitro assay. The most significant example of such an assay, i.e., the functional tube formation assay, using endothelial cells on Matrigel™*, is described below.
The μ-Plate Angiogenesis 96 Well uses the proven “well-in-a-well” feature to avoid meniscus formation in individual wells.
Other advantages include:
90 % less Matrigel is needed, compared to using standard 96-well plates
Allows for efficient imaging as all cells are in one focal plane
Furthermore, only four steps are needed to go from sample preparation to image analysis: