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All-in-One Open Well Formats for IF


Cells are cultured in the classic open well geometry. Fixation, per­meabilization, and staining steps are also done in these wells. After staining and mounting, the sample can be observed through the coverslip bottom using high resolution microscopy.

The ibidi μ-Slide 2 Well, μ-Slide 4 Well, μ-Slide 8 Well, the μ-Slide 2 Well Ph+, μ-Slide 4 Well Ph+, and the μ-Dish 35 mm, high enable standard immunofluorescence protocols, without using coverslips, in an all-in-one open well chamber.

Please refer to the overview table for technical details on these products.

The ibidi Mounting Medium, which is specially designed to work with ibidi µ-Slides and µ-Dishes, prevents photobleaching and allows the sample to be stored for an extended period of time without the need for additional coverslips.

Advantages of All-in-One Open Well Formats

  • Easy and rapid handling
  • No cell transfer
  • No coverslip handling
  • Sterile and ready to use

Limitations of All-in-One Open Well Formats

  • Storage time restricted to several weeks due to gas exchange through the plastic material from which the slides and dishes are made
  • Higher amounts of staining solution needed

Open Slides versus Channel Slides

In open formats, meniscus formation may be prevented by using small coverslips. In channel structures and ibidi's Ph+ µ-Slides, this type of “lid” is integrated into the slide leading to perfect imaging quality without meniscus formation.