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Application Notes

AN 17: Chemotaxis 2D and 3D (PDF)
General protocol for 2D and 3D gel assays with µ-Slide Chemotaxis

AN 23: 3D Chemotaxis Protocol for Non-Adherent Cells in a Gel Matrix (PDF)
Application Note providing a specific example protocol for chemotaxis of dendritic cells in a collagen gel

AN 35: Chemotaxis Assay with Cells in the Reservoirs (PDF)
A detailed protocol for using adherent cells as chemoattractant-producers in the large reservoirs of the µ-Slide Chemotaxis.

AN 24: Chemotaxis of HT-1080 Cells in 2D and 3D (PDF)
Detailed protocol of experimental parameters and example data of HT-1080 cells migrating in 2D and in 3D in collagen I gels

AN 34: Chemotaxis of HUVEC Cells in 2D and 3D (PDF)
Detailed protocol of experimental parameters and example data of HUVEC cells migrating in 2D and in 3D in collagen I gels.

AN 37: Image Shift Correction in Microscopic Time Lapse Sequences (PDF)
Instructions for correcting an externally generated shift of the sample in time lapse images (e.g. in chemotaxis experiments)