Top sellers

Application Notes

AN 08: Cell Culture Coating (PDF)
Making your own coating on µ-slides

AN 06: Trypsination in µ-Channels (PDF)
Removing adherently grown cells from a µ-channel after cultivation

AN 10: Co-Cultivation (PDF)
Application note for co-cultivation of two different cell types in µ-Slide 2 Well Co-Culture

AN 20: Cultivation of Macrophages (PDF)
Cultivation protocol for a murine macrophage cell line in µ-Slide VI0.4 and µ-Slide 8 well

AN 26: Collagen I Gel for 3D Cell Culture (PDF)
Fabrication protocols for collagen I gel (bovine and rat tail) with different cell media

AN 32: Generation of Spheroids (PDF)
Generation of spheroids using the liquid overlay technique

AN 33: Live / Dead Staining with FDA and PI (PDF)
Viability staining of adherent cells, single cells embedded in extracellular matrix, and cellular clusters

AN 38: Western Blot Analysis with Cell Samples Grown in Channel-µ-Slides (PDF)
Detailed protocol for performing cell lysis in channel-µ-Slides and subsequent SDS-Page with Western Blot Analysis

AN 40: Gene Expression Profiling with Cell Samples Grown in Channel-µ-Slides (PDF)
Detailed protocol for performing cell lysis in channel-µ-Slides and subsequent RNA-Isolation with RT-qPCR Analysis

AN39.jpg

AN 39: Cell Proliferation Assay in µ-Slide Angiogenesis (PDF)
A Detailed Protocol for Analyzing the Cell Proliferation Using MDCK Cells for the Example

AN 47: Electrical Stimulation of Cardiomyocytes in µ-Slide I Luer Electrode (PDF)
Typical parameters for the stimulation of cardiomyocytes using the µ-Slide I Luer Electrode as well as a seeding protocol for the cells

AN 12: Avoiding Evaporation (PDF)
Decreasing evaporation during cell cultivation by using the ibidi humidifying chamber Olaf and µ-Slides

AN 01: Gradients Inside µ-Slide I (PDF)
Establishing a concentration profile

AN 04: Lipid Monolayer (PDF)
Description of the uncomplicated and fast preparation of a lipid monolayer on uncoated slides

AN 22: Pixel Size (PDF)
Measuring and calculating the pixel size of microscopic images