ibidi Practical Course:
Setup and Analysis of Chemotaxis Assays

"Thanks for providing such a great practical course on chemotaxis assays. I have subsequently been successful in optimizing a chemotaxis protocol for THP-1 immune cells! I am sure I never would have managed to set up this experiment at all without your help."

Emily Lynes, German Centre for Neuro-degenerative Diseases, Tübingen, Germany

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Course Objectives

The aim of this hands-on course is to learn about the experimental setup and analysis of 2D and 3D chemotaxis assays with the ibidi µ-Slide Chemotaxis. In addition to practicing handling the slide, you will also perform a whole chemotaxis assay that includes sample preparation with cancer cells, live cell imaging, single cell tracking, and data analysis. Also, characteristic parameters of directed and undirected cell migration will be evaluated. During the two-day training, our experts will give you tips and tricks that will be helpful with your own experiments.

Target Group

The course is intended for scientists and technical associates, who have extensive experience with cell culture and sterile working techniques, and want to establish chemotaxis experiments with the ibidi µ-Slide Chemotaxis.

Course Dates

ibidi Headquarters, Gräfelfing near Munich, Germany

  • March 10–11, 2020
  • April 28–29, 2020

>> Register now

Schedule

Day 1 – Start: 10:00 am

Topics

  • Introduction to chemotaxis and cell migration
  • Hands-on: Handling of ibidi µ-Slide Chemotaxis
  • Hands-on: Setup of 2D and 3D chemotaxis assays

End: ca. 5:30 pm

Lunch is included on both days.

Day 2 – Start: 9:00 am

Topics

  • Live cell imaging
  • Evaluation and analysis of chemotaxis assays
  • Hands-on: Single cell tracking and data analysis
    (bring your laptop and a computer mouse!)

End: ca. 4:00 pm