Over more than two decades, ibidi has earned the trust of customers worldwide.
Andi Erega, ETH Zürich, Zürich, Switzerland
I tested the µ-Slide 15 Well 3D and the µ-Slide 18 Well Glass Bottom in my model of bacterial biofilms on special material. The pieces of that material are placed into the wells. I must say that I like the ibidi system a lot! The wells helped me to stain, wash, transfer the pieces and keep them stable during CLSM analysis.
Laboratory of Food Biotechnology
Institute of Food, Nutrition and Health
Luzia Martins Nascentes Melo, University Hospital Essen, Essen, Germany
We have been using the Culture-Insert 2 Well in µ-Dish 35 mm, high and it is a great product. We highly recommend it. Quality and functionality are amazing and the special dish for microscopy is a plus. Our lab is using them for the migration assay of melanoma cells.
Luiza Martins Nascentes Melo
Institute for Tumor Metabolism
University Hospital Essen
Xiaobin Cong, Technical University Munich, Munich, Germany
As for the Collagen Type I, Rat Tail, the instruction manual and also Application Note 26 are really helpful in the 3D cell culture.
The other well-prepared instructions are also very trustworthy. I managed to complete my tube formation assays, learning some skills from your website. Also, many of my colleagues like to use ibidi μ-Slides and Culture-Inserts to facilitate tube formation and migration assays.
Technical University Munich
Ursula Götz, Ludwig-Maximilians-University, Munich, Germany
The products from ibidi are of the best quality and can be recommended without reservation. There is an ideally suited product for every application. The service is very reliable and competent.
Fei San Lee, University of Nebraska, Lincoln, USA
We have been using the Collagen Type I, Rat Tail for more than a year! Collagen Type I from ibidi forms a robust 3D hydrogel after neutralization and is suitable for culturing primary neuronal cells in vitro. We have characterized the gelation kinetics and rheological properties of the hydrogels and are satisfied with the hydrogel properties derived from this product. Also, ibidi's customer service is very responsive and always has the product in stock for quick turnaround time.
Fei San Lee
University of Nebraska
Hellen Ishikawa-Ankerhold, Ludwig Maximilian University München, Germany
I used the µ-Slide Chemotaxis in combination with Collagen Type I, Rat Tail, for the migration of Dictyostelium cells to characterize the position of the centrosome in relation to the cell nucleus during 3D migration.
The preparation of the Collagen and the cells were easy to handle and the insertion into the channel was not difficult either. The instructions and the video demonstrating the preparation were very helpful.
It resulted in a great publication of our work with the use of the ibidi products (Ishikawa-Ankerhold et.al., Cells 2022, Centrosome Positioning in Migrating Dictyostelium Cells).
Ludwig Maximilian University
Stefanie Koch, Dynamic42 GmbH, Jena, Germany
As a life science company, we have had nothing but good experiences with the ibidi cell culture equipment.
Linah Ahad, Robert-Koch-Institute, Berlin, Germany
Hantaviruses specifically infect endothelia in vivo, and the disease is characterized by dysfunction of these endothelia. One of our goals is to identify new drug targets for the treatment of hantavirus disease in the context of host-directed therapeutics. For this, I am working with the ibidi Pump System while using the µ-Slide I Luer 0.6. I really love these ibidi products because they are very easy to handle.
Jonathan Shillingford, University of Michigan, USA
I have recently been experimenting with those RGD printed Bioinert slides (µ-Slides With Multi-Cell µ-Pattern) and they are amazing! I plated mIMCD3 cells and everyone in the lab is literally wowed by the precise array they form. I plan on determining by confocal microscopy if they form discrete cysts, are polarized and, if so, whether treatment with cyst growth agonists affect their size. MDCK cells, at least in my hands, don't look as impressive and my suspicion is that they lay down ECM of their own on top of the bioinert surface and begin to spread out and away from the RGD printed spot.
I do appreciate the technology and scientific thought that went into the perception and manufacturing of this product.
University of Michigan
Ann Arbor, MI
Joyce C.M. Meiring, Utrecht University, Utrecht, The Netherlands
"I used the µ-Dish 35 mm, high Grid-500 Glass Bottom to perform live cell spinning disc microscopy on neurons and U2OS cells, followed by immunofluorescence and imaging on an Airyscan confocal. The gridded dishes made it very easy for me to find the cells on which I performed live cell imaging back after immunofluorescence staining. I am very pleased with the quality of these dishes, but I would caution users to handle them carefully as the coverglass can break."
Joyce C. M. Meiring
Cell Biology, Neurobiology, and Biophysics
Liu Chun, Sun Yan-Sen University, Guangzhou, China
"The Collagen Type I, Rat Tail from ibidi is a very high-quality product. We have been using it for years and it always provides reliable and stable results. We have utilized the collagen as an important component for our new material development, with applications in 3D cell culture and 3D bioprinting. We have also used the collagen successfully for culturing many cell lines and primary cells, including stem cells, tumor cells, and cartilage cells."
Prof. Liu Chun
Key Laboratory of Organ Donation and Transplant Immunology
The First Affiliated Hospital, Sun Yan-Sen University
Wilco Nijenhuis, Utrecht University, Utrecht, The Netherlands
"We tested the µ-Slide 8 Well high Glass Bottom for long-term differentiation and imaging of epithelial cell cultures. We were happy to find that these new high wall microslides seem to prevent infections and are more compatible with long-term cultures."
Center for Living Technologies
Cell Biology, Neurobiology, and Biophysics
Max Urbanczyk, University Tübingen, Germany
"I am working with the µ-Slide I Luer 3D in combination with the ibidi Pump System. Both parts, the chip and the pump, impress by their simple handling. The application of a defined shear force, e.g., for the culture of endothelial cells, makes them a powerful tool for establishing in vitro systems that mimic the native environment of cells."
Institute of Biomedical Engineering
Rita Ribeiro, University of Lisbon, Lisbon, Portugal
"I tested the µ-Slide 18 Well and the µ-Slide 8 Well high. I used them for confocal microscopy. I can say that I am very pleased with these products! Super easy to use and very convenient as we can perform the whole protocol (from cell seeding to microscope reading) in the same chamber, without contamination between wells and with a great image. I will certainly like to use these slide chambers again."
University of Lisbon
Fiona Cunningham, Queen's University Belfast, United Kingdom
The ibidi slides have been great. We have used the ibidi µ-Slide 15 Well 3D Glass Bottom for tube formation assays and the µ-Slide 18 Well chambers for immunostaining of cell cultures. The imaging quality is fantastic!
Queen's University Belfast
Hanna Vuorenpää, Tampere University, Tampere, Finland
"I have tested the µ-Slide 8 Well high. I am especially glad that you now also offer high edge 8 well slides. These are needed in my 3D co-culture applications with a quite thick hydrogel layer. There needs to be room on top of the hydrogel layer for the medium, and this has been an issue with the basic, lower-edge 8 well slides. I have already ordered more of the µ-Slide 8 Well high products."
Eimear Wallace, Explora Biotech, Rome, Italy
"I am planning a cell migration assay to test the bioactivity of our produced VEGF.
I used the silicone Culture-Inserts 2 Well. They worked well and created a nice and uniform cell-free gap in the culture plate well. The 2 well version is the best suited for my current research."
Daniela Conticelli, University of Turin, Italy
"My experience has been very positive. Free samples from ibidi arrived very quickly after my request. I had the opportunity to test the 8 Well Chamber, removable for immunofluorescence, and the result met my expectations. I will definitely consider ibidi for my future purchases of items for applications in microscopy."
University of Turin
Federica Bono, University of Brescia, Italy
"The µ-Slide 8 Well high worked very well, especially for our iPSC-derived neurons. We used them for live cell imaging microscopy, and we had very good results. We are very satisfied with the slide!"
University of Brescia
Jonathan Barroso-González, CNIO (Spanish National Cancer Research Center), Madrid, Spain
"The µ-Slide 8 Well high is a perfect fit for our needs in terms of cell culture and treatments. We will continue to use the slides for our live cell imaging experiments."
CNIO (Spanish National Cancer Research Center)
Diana R. Fonseca, University of Porto, Portugal
"I have tested the µ-Plate 24 Well in a DAPI assay with bacteria and further observed it in an automated fluorescence widefield HCS microscope. The results were very satisfactory. I am really grateful for this."
Diana R. Fonseca
University of Porto
Gaio Paradossi, University of Rome, Italy
"We work on the analysis of the interactions taking place between ultrasound contrast agents, i.e. microbubbles, and cells in the microscale range of displacements under shear rates, which mimic the physiological ones. These studies are becoming increasingly important in ultrasound super-resolution imaging techniques. In our studies involving the interaction of polymer and lipid shelled microbubbles with HUVEC cells, the ibidi µ Slide VI 0.4 channels proved themselves an important tool."
Paper: Journal of Colloid and Interface Science 578 (2020) 758–767
University of Rome
W. Cui, Karolinska Institute, Solna, Sweden
"I love the #1.5 Glass Bottom µ-Slide 18 Well chamber slides and already purchased two boxes, especially for staining small samples. They are very cost-effective for antibodies and reagents. The new µ-Slide 8 Well high chamber slides are very convenient for 3D culture. Our groups used to buy traditional 8-well chamber slides from ibidi, now we have more options."
Deepa Avisetti, Royal Veterinary College, London, United Kingdom
"I found the samples of the µ-Slide15 Well 3D and the Culture-Insert very easy to use and hence I have ordered the wound healing chamber plates and the angiogenesis slides. I have been using cells with extremely slow growth rate because of the overexpressed proteins. The wound healing chambers and the angiogenesis slides do not need many cells, which was particularly very helpful in my case."
Royal Veterinary College
Aparajita Lahree, University of Lisbon, Institute of Molecular Medicine, Lisbon, Portugal
"The free sample of the µ-Dish 35 mm, high Grid-500 that I had requested worked out well for my experiments, and we ordered a box for me to run all my imaging work. I was happy with how quickly the cells I use (HEK-293T and HepG2) got comfortable and presented with a nice distribution. I am quite satisfied with this product, and I will be using it for live imaging microscopy."
University of Lisbon
Institute of Molecular Medicine
Shemona Rattila, Uniformed Services University of the Health Sciences, Maryland, USA
"I have been using the Metavi Labs online platform since 2017 for wound healing image analysis. It has been helping me to analyze huge numbers of images just in less than an hour with simple uploading of the images. In addition to that, the team is ready to help the customers right away with answers to any questions along with any specific optimization that might be required for a particular experiment depending on cell-specific differences. I am gratefully stating that I was able to address my reviewers' comments on some extra experiments, which was possible due to this platform; I was finally able to get the work published. Thanks to Metavi Labs FastTrack AI."
Uniformed Services University of the Health Science
Kristy Wendt, University of Wisconsin-Madison, Madison, USA
"We were really happy with the interface and output from the Chemotaxis FastTrack AI Image Analysis. The software worked great for our galvanotaxis study and was much faster than manually tracking the cells."
University of Wisconsin-Madison,
Philip V'kovski, Institute of Virology and Immunology, Bern, Switzerland
"The slides with the removable chamber were exactly what we needed. With other products in the past, the removal of the chambers was complicated and left some glue on the slide, which made further mounting of the coverslip difficult. With the ibidi products that we have now tested, we are very happy because removing the chambers is very easy. This is why we already placed an order for 3 boxes of these slides."
Institute of Virology and Immunology
Vladimir M. Milenkovic, University Regensburg, Germany
"We tested the µ-Dish 35 mm, high ibiTreat for our human iPSC (induced pluripotent stem cells) derived neuronal cells. Now, we have switched completely from glass coverslips to ibidi ibiTreat plastic ware. This material is so much better for neuronal growth."
Vladimir M. Milenkovic
Andrea Maria Guarino, Telethon Institute of Genetics and Medicine, Pozzuoli, Italy
"I have tried the µ-Slide 8 Well Glass Bottom, and I am completely satisfied! I have used the u-Slide to perform in vivo FRET imaging on HeLa cells transfected with a FRET probe.
The glass bottom is clear and clean (no production residues that I have experienced in similar products of other brands), no scratches, no spots. It was easy to switch from a well to another in the chamber, testing different conditions in the same plate."
Andrea Maria Guarino
Telethon Institute of Genetics and Medicine
Mar Cóndor, University of Leuven, Leuven, Belgium
"I have tested the µ-Slide VI 0.4 ibiTreat, and it worked perfectly. We are going to use the slide for 3D traction force microscopy experiments on individual endothelial cells embedded in reconstituted collagen-based hydrogels."
University of Leuven
Wenting You, University of Maastricht, The Netherlands
"I tested various slides and found that the µ-Slide 8 Well worked very well. I use the slides for confocal microscopy imaging. My cells are very easy to detach from glass bottom cell culture slides. Therefore, I tried the slides with a Polymer Coverslip bottom. The cells adhere to the cell culture slide very well, and I can get high-resolution images. I have already ordered more polymer cell culture slides from ibidi."
University of Maastricht
Juliane Proft, IOCBTech, Prague, Czech Republic
"I have worked with ibidi cell culture dishes to cultivate primary hippocampal neurons. The neurons were prepared from mice aged 4 months to 2 years. Imaging was done on a Nikon A1 confocal microscope. I had the feeling "my" cultured neurons liked the ibidi µ-Dishes better than other commercially available dishes. I highly recommend the ibidi µ-Dishes."
Michele Chiappi, Imperial College, London, United Kingdom
"I have tested the slides with 8 wells and detachable soft silicon wells (8 Well Chamber, removable). They are amazingly easy to use and then easy to mount a coverslip and do confocal microscopy. I love them."
Johanna Hol Fosse, Norwegian Veterinary Institute, Oslo, Norway
I have tested the µ-Slides 8 Well on adherent fish cell lines, originally isolated from salmon head kidney (ASK and SHK cells) and have been very happy with them. The ASK cells preferred the ibiTreat slides, while the SHK cells grew well on both ibiTreat and Collagen IV. I also tested the ASK cells in a µ-Slide 18 Well - Flat and that worked well, provided that the medium (L-15, 10% FCS) was changed at least every other day. Cells were seeded 5,000/well in the µ-Slide 18 Well - Flat and 50,000/well in the µ-Slide 8 Well. I will place an order within the next few weeks."
Johanna Hol Fosse
Norwegian Veterinary Institute
Jana Kudova, Czech Academy of Sciences, Brno, Czech Republic
"I had a chance to test the Culture-Insert 2 Well in a µ-Dish with FaDu cells (a hypopharyngeal carcinoma cell line), and I have to say that it works perfectly. Before, I did a scratch assay using pipette tips. It was very difficult and the reproducibility was low. When using the ibidi Culture-Insert, all issues disappeared. I have recommended your product to other colleagues and we plan to use it as a routine method in our lab."
Czech Academy of Sciences
Tan Hop Huynh, University of Basel, Biozentrum, Basel, Switzerland
"I checked the interaction of bacteria and host cells by monitoring the fluorescence signals in the time-lapse experiment. Your µ-Slide 18 Well Flat did a great job to bring properties and clear results."
Tan Hop Huynh
University of Basel
Yuki Manabe, Kyoto University, Graduate School of Agriculture, Kyoto, Japan
"Thank you for your great product µ-Slide 8 Well. I think it is the best for obtaining fluorescent images of adherent cells such as RAW264 and RBL-2H3 using oil immersion microscopy. Thanks to this slide, we can get various good results."
Graduate School of Agriculture
Michelle Somerville, Cardiff University, Cardiff, United Kingdom
"We have tested the µ-Slide 15 Well 3D and the µ-Slide 8 Well and have found them to be great products. The slides are being used for imaging fluorescently labelled organoids using confocal microscopy and we will continue to use them. I have already recommended them to a lab colleague and will continue to recommend them to others."
Milena Leseva, The Stephan Angeloff Institute of Microbiology, Sofia, Bulgaria
"The 12 Well and 8 Well Chambers, removable worked very well. We used them for fluorescence staining for H4K16ac in non-adherent, neutrophil cells. We treated the cells with different compounds that are expected to change this modification.
Overall, we are extremely happy with how easy it is to use the cell culture chambers, especially when you have cells with different treatments. We liked the fact that the chamber format minimizes the volume of antibody that we required in order to get a good signal."
The Stephan Angeloff Institute of Microbiology
Francesca Sartor, Ludwig-Maximilians-University, München, Germany
"I tested the dishes with glass bottom for fluorescence microscopy applications. We used U20S cells, plated on the ibidi Glass Bottom Dish 35 mm, and performed time-lapse imaging. I found the quality of the samples very good. We could get sharp microscopy pictures."
Paola Antonello, Institute for Research in Biomedicine, Bellinzona, Switzerland
"I tested the μ-Slide 15 Well 3D to study the chemokinesis of lymphoma cells in a 3D migration using collagen as a matrix in the presence of the chemoattractant. I used these slides to test different concentrations of bovine collagen, in order to optimize my protocol. The slides worked perfectly. I tested the rate of chemokinesis in an ImageXpress confocal microscopy for 6 hours and obtained a very good resolution with 4X magnification using phase contrast."
Institute for Research in Biomedicine
Patrick Nijman, Technical University Delft, The Netherlands
"I am using various types of the µ-Slide I Luer under flow conditions to study Schistosomiasis haematobium eggs in urine flow for diagnostic purposes. I am very satisfied with the product! These slides have been of great help so far. Actually, my supervisor and I are thinking of maybe ordering a large amount of Luer slides (>100) in the future."
Technical University Delft
Christian Ploner, Medical University of Innsbruck, Austria
"We tested the µ-Plate 96 Well for long-term, in-vitro differentiation experiments with human adipose derived stem cells (hASC). In our hands, this plate type is the only one (compared to other optical plates) where the cells differentiate properly, and which was qualified for high throughput screening with a confocal scanning microscope."
Medical University of Innsbruck
Serene Gwee, National University of Singapore, Singapore
"I started using the Glass Bottom chambered slides with my time-lapse imaging of zebrafish embryos and they have been very useful and applicable for my experiments."
Sze Ling Gwee
National University of Singapore
Department of Biological Sciences
Lee Shu Ying, National University of Singapore, Singapore
"As a microscopy core facility, we have to recommend the most suitable imaging plates to our users for their experiments. The µ-Slide 8 Well is our best seller amongst the other wonderful platforms that ibidi carries.
We had multiple experiments where the cells/bacteria were not adhering well to glass coverslips, but grew perfectly on the ibiTreat surface. Confocal images turned out great as well. Our users also love how the cover fits snugly for ease of handling."
Lee Shu Ying
National University of Singapore
Yong Loo Lin School of Medicine
Susanne Proksch, Medical Center–University of Freiburg, Freiburg, Germany
"The µ-Slide Chemotaxis made it possible to study stem cell-mediated chemoattraction of tissue cells, which was realized in a co-culture experiment. I was thrilled with the deliberate system and the convenient all-in-one package supplied by ibidi, including training classes and online courses on how to set up the experiment and how to analyze the data, all with perfect individual customer support. From my point of view, the method is pretty fancy and the µ-Slide Chemotaxis system is very sophisticated, allowing for in vivo-like insights into cell behavior for high-impact publications."
Medical Center–University of Freiburg
Rachid Rezgui, New York University Abu Dhabi, Abu Dhabi, UAE
"We have successfully tested the µ-Slide 4 Well and 8 Well Glass Bottom. We are very satisfied with these slides. First of all, the fast delivery allows us to be more flexible when we plan our experiments. We tried Fluorescence correlation spectroscopy to study the interaction of Cell Penetrating Peptides with small interfering DNA and obtained great results. As FCS measures the concentration in the focal volume, it is extremely sensitive to surface changes. As we used glass bottom dishes, we were able to achieve a very small focal volume for maximum precision. We also used them for confocal imaging on Mouse Embryonic Fibroblasts (GSK3-KO MEF) to study molecular motors and obtained great images on a Leica SP8 with Hyvolution."
New York University AbuDhabi
Ali Alhoshani, King Saud University, Riyadh, Saudi Arabia
"The ibidi Culture-Insert 2 Well 24 plate was unbelievably helpful when I wanted precise and consistent results for wound healing assay. It saved my lab so much time and effort. The web-based quantitative image analysis feature is a huge plus. Thank you for such useful products. "
King Saud University
Department of Pharmacology and Toxicology
Contu Raluca, National Center of Neurology and Psychiatry, Tokyo, Japan
"We often use ibidi glass bottom dishes for live imaging of cultured cells. We were able to get very good images of protein delocalization, as well as of nucleic acids taken up by living cells. The optical quality of the bottom of the dishes is extraordinary. Actually, two of our papers for which we used ibidi µ-Dishes and μ-Slides were published in 2017:
Contu et al. 2017 J Cell Sci. http://jcs.biologists.org/content/130/17/2843.long
Takahashi et al. 2017 RNA Biology http://www.tandfonline.com/doi/full/10.1080/15476286.2017.1302641"
National Center of Neurology and Psychiatry
Pierre-Olivier Bagnaninchi, MRC Centre for Regenerative Medicine, University of Edinburgh, UK
"ECIS made it happen: without impedance sensing we will never have been able to look at barrier function in the liver in addition to pure toxicity. This unique feature of the ECIS Zθ allowed us to shed a new light on how the common painkiller paracetamol (acetaminophen) causes liver damage." (http://www.nature.com/articles/srep37541)
MRC Centre for Regenerative Medicine
University of Edinburgh
Astrid König, Senckenberg Museum of Natural History, Görlitz, Germany
"We use the μ-Slide 8 Well Grid-500 for tracking experiments of tardigrades and nematodes. We are really thrilled about the product. The scaling of the slide helps us very much in evaluating the movement of the animals during the experiment."
Please find more details at:
Senckenberg Museum of Natural History
Max-Joseph Kraus, Praxis Geiselgasteig, Grünwald, Germany
"I really love these slides! They are the best that happened to my research workflow. I mainly use the removable 12-well chambers for the high resolution dark field microscopy of platelets and they work excellent for me."
Henrike Rebl, University Medicine, Rostock, Germany
"I have tested the Culture-Insert 2 Well and 3 Well and they worked quite nicely. I will definitely use the ibidi Culture-Inserts and µ-Dishes again because they are so easy to use and they work great for fluorescence microscopy."
Petra Weber, Aalen University, Optical Engineering, Aalen, Germany
"We have tested the Removable Chambers (3 Well and 8 Well) and were very happy. Due to our very sensitive TIRF microscopy with an upright microscope we need appropriate glass slides with removable chambers. We tested adherent HeLa cells to detect Förster Resonance Energy Transfer between EGFR-CFP and Grb2-YFP with Fluorescence Lifetime Imaging. In particular for the activation or inhibition of intracellular signaling pathways, we need the subdivisions on the object slide. It was very easy to remove the chambers. Thank you very much for the possibility to test the chambers for free. I am sure I will use them again."
Aalen University, Optical Engineering
Daniel Kummer, ZMBE, Institute for Medical Biochemistry, Münster, Germany
"I tested the 8 Well Chamber, removable, and was very happy with the results. The system worked flawlessly. The cells grew very well and the immunofluorescence stainings were of good quality. In general, our institute has always had good experiences with ibidi products. It was the same with the 8 Well Chamber, removable. I have already recommended it to my colleagues."
ZMBE, Institute for Medical Biochemistry
Sofia Oke, University of Toronto, Department of Immunology, Toronto, Canada
"I have had the chance to try the µ-Slide 8 Well Grid-500 and have been very impressed. I have been using the slides to perform assays with 3D organoid cultures. The µ-Slide 8 Well Grid-500 was very useful and easy for me to monitor my organoids as they grew. I was performing an assay where I irradiated my organoids and monitored recovery. I am very impressed with these products and will be sure to keep in touch if I once again need them in the months to come.
University of Toronto, Department of Immunology
Gisa Gerold, TWINCORE – Center for Experimental and Clinical Infection Research, Hannover, Germany
"We tested the Culture-Inserts 2 Well and now use them successfully on human hepatoma cells (Lunet N3 hCD81 cells, which are derived from Huh-7.5 cells). We are very satisfied with the ibidi products and with the fast delivery of the test chambers."
TWINCORE – Center for Experimental and Clinical Infection Research
Madeleine Zerbato, Brigham Regenerative Medicine Center, Cambridge, USA
"The ibidi µ-Slide 4 Well and µ-Slide 8 Well that I am working with are actually the best products I have tested so far for cultivating D3 mouse embryonic cell lines. They attach well to the treated slide (ibiTreat µ-Slides), and the resulting morphology is very similar to the one they have on regular plastic. That is something very unique to those microscopic slides, since D3 mESCs do not attach to glass and form embryoid-like structures. I tested other non-glass slides from other manufacturers but the ibiTreat µ-Slides are the only ones that give me a physiological-like morphology."
Brigham Regenerative Medicine Center
Des Field, University College Cork, Ireland
"I'm glad to say that the ibidi plates were ideal for our purposes. I am working with bacteria (Staphylococci and Pseudomonas) that form biofilms on surfaces. They easily form biofilms in regular 96 well polystyrene microtiter plates but these cannot be visualized by confocal microscopy. We are using combinations of antibiotics to eradicate these biofilms and wished to use live/dead fluorescence staining and confocal microscopy to visualize the results. We set up the experiment using the ibidi µ-Plate 96 Well and we were able to observe perfectly the results of our experiments. We will be hoping to upscale this type of work in the near future (given the quality of the results we have achieved) and so will hope to be ordering more of these microtiter plates in the near future."
University College Cork
Barbara Fogli, Medical University Innsbruck, Austria
"I tested the µ-Slide 4 Well for the culturing of mice DRG neurons. I found them particularly useful for my experiments, since they allow me to have different conditions within the same slide, and to directly do staining and microscopy within the same slide.
After testing them, I decided to order a box of the µ-Slide 4 Well Ph+, because the plastic layer in the middle of the wells allows me to have a more homogeneous distribution of the neurons, which is optimal for microscopy."
Medical University Innsbruck
Jessica Davis, University College Dublin, Ireland
"I used the µ-Slide 8 Well in the glass version and in the plastic version with ibiTreat. They were perfect for my experiment—I was performing live cell imaging on 3D cell culture. I found that the Matrigel adhered very well to both slides. The coverslip thickness of the base resulted in superb imaging. Additionally, I noticed that the lid was very secure compared with other chamber slides I have used in the past. I am very happy with the product and have recommended it to other colleagues."
University College Dublin
Catarina Leite Pereira, University of Porto (INEB), Porto, Portugal
"I have done some experiments using the µ-Slide 2 Well and µ-Slide 4 Well for static culture ex vivo, and imaging of intervertebral discs at the IN Cell Analyzer. They work perfectly! Thank you very much, these slides are really very useful, since I have been struggling for some time trying to find the appropriate support to visualize this kind of sample."
Catarina Leite Pereira
University of Porto (INEB)
Renate Gehwolf, Paracelsus Medical University, Salzburg, Austria
"We are currently using the Culture-Insert to perform wounding experiments with somatic stem cells, and also the 2D chemotaxis slides to study the migratory behavior of these cells. We are very happy with the products, because they are user-friendly, especially for wounding assays, as we do not want to do scratching because of the inevitable high variability. However, with the ibidi Culture-Inserts, we can perform highly reproducible experiments. We will definitely use them again."
Institute of Tendon & Bone Regeneration
Paracelsus Medical University
John Fassett, University of Graz, Austria
"The µ-Slides 8 Well are great! I really like the ones with the grid. We were able to use them for the live cell imaging of transfected GFP and RFP fusion proteins and could see some really good images."
University of Graz
Maros Mastrak, Medical University Innsbruck, Austria
"I tried your mounting medium, which worked really great. I had an older one from a different manufacturer, but it caused very high background levels. However, with the ibidi Mounting Medium, I was able to save at least half of the exposure times and gain much clearer results."
Medical University Innsbruck
Kenneth Martin, University College Cork, Ireland
"Thank you for the great products that you have developed over the years. The ibidi Pump System has immeasurably helped our research. We have recently published a paper in "Stem Cells" that uses your flow system, the µ-Slide I Luer, and the µ-Plate 96 Well very effectively. It seems to me that ibidi are constantly coming up with clever and useful products, and I like to keep an eye on what interesting things are coming out next from them."
University College Cork
Stefanie Kirchberger, Children's Cancer Research Institute, Vienna, Austria
"We tested the µ-Slide 2 Well and µ-Slide 8 Well in the uncoated version for the confocal microscopy of fish larvae. Both types of slides worked fine on our confocal microscope. We have already ordered another bunch of those slides for the lab!"
Children's Cancer Research Institute
Tim Sanchez, Harvard University, Cambridge, USA
"For my research, I need to take time-lapse images of mouse embryos developing from the 1 cell stage all the way to 5-day blastocyst. Anyone who has worked with mouse embryos knows they are incredibly sensitive and delicate, so we required an extremely stable environment over several days. I struggled for months with a different system, but the ibidi Heating Stage worked very quickly. I was immediately impressed with the quality. Calibration with the included thermocouple was easy and accurate. Now I get consistent development every time, and the percentage of embryos developing to blastocysts is the same as the control incubator! Terrific product, thanks ibidi."
Emily Lynes, German Centre for Neurodegenerative Diseases, Tübingen, Germany
"Thanks for providing such a great practical course on chemotaxis assays. I have subsequently been successful in optimizing a chemotaxis protocol for THP-1 immune cells! I am sure I never would have managed to set up this experiment at all without your help."
German Centre for Neurodegenerative Diseases
Jing Zhiao Garland, University of Cambridge, United Kingdom
"Thank you very much for organizing the Training Course. It was very helpful. We all enjoyed the course very much and I felt I was energized after attending this training. A big special thank you for letting me rediscover the great value of the ibidi website."
Jing Zhao Garland
University of Cambridge
Department of Medicine
Ivan Bedzhov, University of Cambridge, Gurdon Institute, Cambridge, United Kingdom
"We looked for an imaging-compatible material that supports embryo attachment. This led us to establish that we can successfully grow and image mouse embryos on ibiTreat μ-Plates 24 Well and µ-Slides 8 Well from ibidi, which have optical properties similar to glass, and allow efficient blastocyst attachment. Thus, ibidi slides and plates are now an indispensable part of our in vitro culture methodology."
University of Cambridge
Anuradha Vajjala, Nanyang Technological University, Singapore Centre on Environmental Life Sciences Engineering, Singapore
"To study the ultra structure of Streptococcus pyogenes biofilm grown on mouse embryonic fibroblasts, I used the ibidi Treat 35 mm μ-Dish to optimize the right conditions of biofilm growth. Simply awesome! It has made life so much simpler for me. Tissue culture combined with microscopy is so much easier now. The dishes are very user-friendly too. I have used the ibidi products to get very good quality images. I am really happy with the products!"
Nanyang Technological University
Singapore Centre on Environmental Life Sciences Engineering
Randy Van Der Ploeg, St. Boniface Research Centre, Winnipeg, Manitoba, Canada
"We are pleased with the ibiTreat 35 mm culture dishes. I have given some of your product to one of our grad students to try and he is amazed with the robust axonal outgrowth that he finds only after one day of seeding cells. I have tested other dishes from your competitors and I did not get the reproducibility in growth characteristics like the ibiTreat µ-Dish.
The 35 mm dish holds up wonderfully to automated scanning over a wide scan area (essentially 98% of the growth surface area) which demonstrates the optical clarity and focal stability of the surface over such a wide area (1296 scan fields per well). I look forward to using more of the ibidi product lines in the future."
Randy Van Der Ploeg
Division of Neurodegenerative Disorders
St. Boniface Research Centre
Kaela Varberg, Indiana University, School of Medicine, Indianapolis, USA
"The µ-Slides 15 Well 3D created by ibidi have significantly increased the efficiency and accuracy of the angiogenesis assays in our lab. Using the ibidi slides not only reduces the amount of time spent capturing images, but it also reduces the time it takes to quantify the data since the structures are in the same focal plane. Another added bonus is the reduced amount of matrigel required for each experiment which saves money!"
School of Medicine
Shane R. McSweeney, Cardiovascular Division, BHF Centre of Research Excellence, School of Medicine, King's College London, United Kingdom
"The µ-Slide I Luer facilitates the culture of endothelial cells under defined complex flow patterns. We are particularly interested in the effects of oscillatory shear stress on redox signaling pathways, and the ibidi Air Pump and Fluidic Units are ideal systems for this purpose. The µ-Slides are convenient for both live and fixed cell imaging as well as the collection of protein and RNA samples and other cell analyses. The system has allowed us to easily investigate physiological shear patterns in vitro without a background in engineering."
Shane R. McSweeney
BHF Centre of Research Excellence
School of Medicine
King's College London
Judith Cathcart, Advanced Optical Microscopy Facility, University Health Network, Toronto, Canada
"The ibidi chambers (Culture-Inserts) we used in our wound healing assays helped to keep the cell free area clean and consistent in size without damaging the ECM or leading edge of the cell fronts, making analysis of results more manageable and comparable. They're convenient and easy to use and their small size helps to conserve precious cells and culture materials. We'd certainly use them again."
Advanced Optical Microscopy Facility
University Health Network
Jason W. Bjork, PhD, 3M Corporate Research Materials Laboratory, St. Paul, USA
"The ibidi Culture-Inserts for wound healing assays provide a new line of testing for us. We wouldn't do traditional scratch assays at all because of the inherent variation, but with the new inserts we're able to provide many images to support our research. Our results are repeatable and reliable regardless of which researcher conducts the assay."
Jason W. Bjork
3M Research Materials Laboratory
Ross Flockhart, PhD, Stanford School of Medicine, Stanford, USA
"The ibidi cell migration Culture-Inserts enabled me to perform highly reproducible and quantitative migration experiments. Since physical scratching isn't required, uniformity is maintained and adverse wounding responses are not an issue.
The online quantification tool WimScratch for the assay is also fantastic and it yields quantitative data from the images extremely quickly with little effort required. I'd definitely recommend it for migration studies."
Ross Flockhart, PhD
Stanford School of Medicine
Department of Dermatology
PD Dr. Peter J. Hanley, University of Münster, Münster, Germany
"In the past 5 years, we have extensively used the ibidi µ-Slide Chemotaxis 2D to perform time-lapse images of primary mouse macrophages migrating in a chemoattractant gradient. We are most grateful that this device was developed, otherwise it would have been technically difficult to perform robust chemotaxis assays over long time periods (in our case, up to 14 h). By adding a little food coloring dye to the chemoattractant solution one can observe that the dye requires days to equilibrate with the opposing reservoir. Gradient kinetics can be measured by substituting the chemoattractant for a fluorescent dye (with similar molecular weight) and taking serial confocal fluorescence images in the observation area, the narrow channel which connects the two reservoirs."
PD Dr. Peter Hanley
University of Münster
Institute of Molecular Cell Biology
Loïc Dupré, INSERM, Toulouse, France
"The ibidi µ-Slides Chemotaxis 2D and 3D have been the key to my research on lymphocyte chemotaxis. These slides have allowed me to track and visualize directional motility in lymphocytes. The generated chemokine gradients are stable for over 24 hours and the viability of the cells is amazingly good, allowing for measurements of chemotaxis over prolonged time frames.
I am also using the ibidi Gas Incubation and Temperature Control Systems, which are robust and guarantee perfect stability. Very importantly, the product developers at ibidi provide a great support for the implementation of their systems. Thanks to ibidi for developing cell imaging products of such great quality!"
Loïc Dupré PhD
INSERM UMR 1043
Purpan University Hospital
Sam Noppen, Rega Institute for Medical Research, Leuven, Belgium
"I used the ibidi µ-Plate 96 Well 3D for bioluminescence measurements. The plate performed exceptionally well for this specific application, as it reduced my sample volume drastically and the optical quality was just superb."
Rega Institute for Medical Research, KU Leuven
Joanne Marrison, University of York, UK
"I plated and transfected cells on the ibidi µ-Slide 8 Well alongside the regular chambers we normally use and they were very good. In fact, I particularly liked the lids which seem more secure than our regular 8-well chambers and the fact that they are 'slide' sized with space for writing a label. Another bonus was the individual packaging which gives me greater confidence on sterility particularly when not all the chambers in a pack of 8 are used at once. So all in all great."
Imaging and Cytometry Laboratory (B/K051)
Bioscience Technology Facility, Department of Biology
University of York, York
Matthew Taylor, PhD, Princeton University, Princeton, USA
"The 35 mm µ-Dish is invaluable for high resolution imaging performed in support of my work. The optical plastic is fantastic substrate for building compartmentalized neuronal cultures. Thank you for making great products."
Department of Molecular Biology
Dr. Esther G.L. Koh, National University of Singapore, Singapore
"ibidi made it much simpler for me to prepare cells for confocal microscopy and live-cell timelapse microscopy. Cells that attached poorly to glass grew better on ibidi µ-Slides/Dishes. The µ-Slide 15 Well 3D allowed me to minimise the amount of cells and reagents required for an experiment."
Dr. Esther G.L. Koh
Head, Advanced Imaging Laboratory
Life Sciences Institute Immunology Programme
National University of Singapore
Centre for Life Sciences
Dr. Nynke van den Akker, Maastricht University, Maastricht, The Netherlands
"We've been working with the ibidi Pump System and Labware for over 5 years now and have recommended it to numerous colleagues as well. The plastics are ideal for cell culture and all forms of imaging, including automated imaging (easy to focus) and all sorts of fluorescence (clear, optically superb plastics). In faact, the ibidi Pump System makes the endothelial cell under flow the default of our lab! And above all, the technical support is always fast and efficient."
Dr. Nynke van den Akker
Department of Cardiology
Owen McCarty, PhD, Oregon Health & Science University, Portland, USA
"The ibidi platforms have opened new possibilities for our group to utilize small volumes of blood to characterize the cell biology of platelets and white blood cells. Moreover, we have greatly benefited from collaborating with the technical representatives at ibidi to discuss ways to multiplex the ibidi platforms to address our experimental questions."
Owen McCarty, PhD
Department of Biomedical Engineering
Oregon Health & Science University
James J. Faust and David G. Capco, Arizona State University, Tempe, USA
"ibidi has made the tedious process of correlated light- and scanning electron microscopy remarkably simplified with their µ-Dishes 35mm, high Glass Bottom Grid 500. This product combines all of the essential elements for high quality imaging: optically flawless glass essential for high resolution light microscopy, media reservoirs suited for a variety of imaging systems, and a grid system to rapidly relocate specimens in the scanning electron microscope. This product has made the difficult easy."
James J. Faust and David G. Capco
Molecular and Cellular Biosciences
Arizona State University
Dr. Thomas A.J. McKinnon, Imperial College London, UK
"I work with the ibidi slides because they are superior to any other product of this kind on the market. They are easy to use, give consistent results, economical and are suitable for a wide range of applications. The flow slides have without a doubt transformed my labs research and made many new experiments possible. Well done ibidi! I am indeed extremely happy with the ibidi products."
Dr. Thomas A.J. McKinnon BSc, PhD
Post Doctoral Research Associate
Department of Hematology
Imperial College London
Prof. Dr. Markus Sauer, University of Wuerzburg, Germany
"ibidi slides are compelling!
We tested the open μ-Slides with 8 wells for live-cell super-resolution imaging by dSTORM with SNAP-tags. The quality of the super-resolved images was impressive."
Prof. Dr. Markus Sauer
Biotechnology & Biophysics
Prof. Dr. Stefan Zahler, University of Munich, Germany
"Using the µ-Slide 15 Well 3D from ibidi is the only possibility that I know for achieving a consistently good optical quality in the tube formation assay, and saving Matrigel at the same time.
In comparison to other systems, the heating stage from ibidi delivers superior thermal stability and enables you to work with high humidity (>80%). This is indispensible for long-term studies and not possible with other systems."
Prof. Dr. Stefan Zahler
Munich Center for System-Based Drug Research
Jason Jacoby, University of Illinois, USA
"Actually, ibidi dishes have single-handedly revolutionized my science. The cells that I use (horizontal retinal cells from fish) are extremely intolerant to being plated on any form of glass, even if the glass is treated with several different compounds. Because of this issue, I was only able to perform fluorescent microscopy on upright confocal microscopes; they allowed us to plate our cells on plastic dishes and image from above. Now, with the ibidi dishes, we are able to use better quality inverted microscopes and higher powered objectives. Our results have proven strong, and we have mentioned the power of ibidi products in our most recent publication.
I am so happy that I learned of your great products through a colleague. Microscopy will never be the same for our laboratory. So far I have purchased 3 boxes of 35mm ibiTreat high dishes. Thank you very, very much."
Jason Jacoby PhD Candidate
Laboratory of Neuroscience
University of Illinois at Chicago
Prof. Dr. Klaus Palme, University of Freiburg, Germany
"The cooperation with ibidi made it possible to provide cell culture systems with a unique optical quality. The use of ibidi slides allowed for image acquisition in the necessary optical resolution, so that development processes in living proteoplasts of the model plant Arabidopsis thaliana could be explored. The µ-Slides allow monitoring of developmental processes in highest resolution. This enables us to use genetic techniques for recording and controlling the necessary processes of organ development."
Prof. Dr. Klaus Palme
Institute for Biology II
Freiburg Institute for Advanced Studies FRIAS – School of Life Sciences