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Microscopy Techniques and Culture Surfaces:
Find the Perfect Match

Compatibility of Different Surfaces With Light Microscopy Techniques

Standard Polystyrene
Plates and Dishes
Phase Contrast
++
++
++
Differential Interference Contrast (DIC)
++
++
-
Widefield Fluorescence
++
++
-
Confocal Microscopy
++
++
-
Two-Photon and Multiphoton Microscopy
++
++
-
Fluorescence Recovery After Photobleaching (FRAP)
++
++
-
Förster Resonance Energy Transfer (FRET)
++
++
-
Fluorescence Lifetime Imaging Microscopy (FLIM)
++
++
-
Total Internal Reflection Fluorescence (TIRF)
+
++
-
Super-Resolution Microscopy
+
++
-