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Method Comparison:
Using LifeAct for F-Actin Visualization in Living Cells

Method Suitable for 1        
Cell lines
+++
+++
+++
+++
Primary cells
+
++
+++
+++
Non-mammalian cells
-
+
-
-
Transfection Stability        
Transient
Yes
Yes
Yes
No
Stable
Yes
No
No
Yes
Protocol and Analysis        
Protocol simplicity
+++
++
+++
+++
LifeAct readout start
1–2 days
few minutes
1–2 days
1–2 days
LIfeAct readout duration
several days
(infinity if stable)
up to 1 day
several days
several days
(infinity if stable)
Signal intensity
+++
+
+++
+++
Extra Benefits        
Prevention of overexpression artefacts
-
+++
+
+
Prevention of viral response
+++
+++
-
-
Biocompatibility of transfer method
+
+++
++
++
Actin functionality
+++
+++
+++
+++
Safety Consideration
Biosafety Level
BSL1
-
BSL2
BSL2


1 Not all of these combinations have been tested in all indicated cell types. We encourage you to use this table as a guideline to understand how the LifeAct system can be optimally applied.