An open µ-Slide (chambered coverslip) with 8 wells for use in...
A µ-Slide used to investigate angiogenesis in tube formation assays....
Used to investigate chemotaxis of fast or slow migrating adherent cells...
Channel slides with different heights, volumes, and coatings specially...
To optimize the F-actin visualization in your experimental setup, ibidi has developed a broad LifeAct portfolio:
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LifeAct Plasmids: A range of plasmids for transient or stable transfections of various cell types (e.g., using the Torpedo DNA transfection reagent); useful for brilliant visualization of F-actin. |
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LifeAct Adenoviral Vectors: Ready-to-use adenoviral vectors for efficient F-actin transduction, especially suitable for studies in difficult-to-transfect cells. |
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LifeAct Lentiviral Vectors: Lentiviral vectors for easy generation of stable LifeAct-expressing cell lines with unrestricted actin functionality. |
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LifeAct-TagGFP2 Protein: A recombinant protein for remarkably fast staining and immediate functional analysis of F-actin in living and fixed cells. |
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LifeAct Stable HT-1080 Cell Line: A stable, LifeAct-expressing human fibrosarcoma cell line with full actin functionality for direct use in cell-based assays. |
Find the most suitable LifeAct product for your application below.
To readily achieve high imaging performance, transfection or transduction of LifeAct can be easily done in ibidi’s μ-Slides and μ-Dishes. Discover more in our detailed Application Notes.
Listen to ibidi’s recorded webinar "LifeAct Actin Marker: A New Tool for the Visualization of Dynamic Cellular Processes" or watch our movie, “LifeAct: Actin Marker for Live Cell Imaging (MV19)”.
Method Suitable for 1 | |||||
Cell lines |
+++
|
+++
|
+++
|
+++
|
|
Primary cells |
+
|
++
|
+++
|
+++
|
|
Non-mammalian cells |
-
|
+
|
-
|
-
|
|
Transfection Stability | |||||
Transient |
Yes
|
Yes
|
Yes
|
No
|
|
Stable |
Yes
|
No
|
No
|
Yes
|
|
Protocol and Analysis | |||||
Protocol simplicity |
+++
|
++
|
+++
|
+++
|
|
LifeAct readout start |
1–2 days
|
few minutes
|
1–2 days
|
1–2 days
|
|
LIfeAct readout duration |
several days
(infinity if stable) |
up to 1 day
|
several days
|
several days
(infinity if stable) |
|
Signal intensity |
+++
|
+
|
+++
|
+++
|
|
Extra Benefits | |||||
Prevention of overexpression artefacts |
-
|
+++
|
+
|
+
|
|
Prevention of viral response |
+++
|
+++
|
-
|
-
|
|
Biocompatibility of transfer method |
+
|
+++
|
++
|
++
|
|
Actin functionality |
+++
|
+++
|
+++
|
+++
|
|
Safety Consideration | |||||
Biosafety Level |
BSL1
|
-
|
BSL2
|
BSL2
|
1 Not all of these combinations have been tested in all indicated cell types. We encourage you to use this table as a guideline to understand how the LifeAct system can be optimally applied.