Principles and Basics of Light Microscopy for Cell-Based Assays
During the last decade, the imaging of cultured, or primary, cells has played a major role in helping to understand biological processes. Cell-based assays use light microscopy as one of the most dominant parameters in the visualization of multi-parameter data sets.
In this webinar we will present the basics of microscopy starting with the inner workings of modern microscopes. We will also discuss the optical properties of glassware and Petri dishes, including the underlying physics. Finally, you will learn about the principles of fluorescence microscopy techniques and modern live cell imaging, using specially designed ibidi µ-Slides and µ-Dishes.
This webinar will focus on the following topics:
- Basics of light microscopy (e.g. optical properties and cover slips)
- Practical aspects and applications (e.g. Köhler illumination and contrast techniques)
- Fluorescence microscopy
- Live cell imaging applications
Elias Horn, ibidi GmbH
Elias Horn studied biotechnology at the University of Applied Science, Mittweida. Since 2004, he has been working on the development of customer-specific slides, for in vivo cell culture experiments, in the Research and Development department at ibidi. His main focus is the design and implementation of migration and chemotaxis assays.