Cell Cultivation Under Flow – New Insights and Applications
Certain cells, such as the endothelial cells in blood vessels or renal epithelial cells, are constantly exposed to shear stress in vivo. Experimentally, flow conditioning of the cells is achieved by perfusing medium through cultivation chambers, and by maintaining these flow conditions over a long period of time, lasting from hours, up to days.
This webinar will provide an overview of the function of endothelium as a barrier between the blood stream and the tissue beneath. It will also introduce various perfusion applications and experimental setups. In addition, the physical background of flow systems will be explained, as well as the impact of these factors on cells, regarding morphology, physiology, and function.
There will be a special focus on new applications that use the ibidi Pump System, such as impedance measurements, pulsatile flow, and the addition of substances to the flow system. The effect of shear stress parameters on cells will be elucidated by new internal experimental results, as well as by other publications.
Learn more about:
- New applications for the ibidi Pump System, such as:
- impedance measurements under flow
- pulsatile and oscillating flow
- solution exchange
- Physical values that impact cell culture, such as:
- flow chamber geometry and shear stress
- influence of surface characteristics
- How to choose a suitable system for your application
Helga Wagner, ibidi GmbH
Helga Wagner studied Biology at the University of Regensburg. Since 2008 she is working in Research & Development of custom specific slides for functional cell based assays at ibidi GmbH. Her focus is on the design and implementation of tube formation assays and flow assays.