Cell Cultivation Under Flow - Introduction
Several adherent cell types are exposed to shear stress conditions in vivo, e.g. endothelial cells in a blood vessel. Simulating these perfusion conditions provides a more physiological environment and induces biochemical responses in the cell physiology.
This webinar provides an overview of various perfusion applications and experimental setups. The physical background of flow systems will be explained as well as the impact of these factors on cells and the choice of experimental setups.
The webinar will demonstrate the setup of a long term experiment, i.e., one lasting over several days, and provide microscopic pictures of endothelial cells. It will also highlight important experimental details and provide advice on how to troubleshoot through possible obstacles.
Learn more about:
- How to choose a suitable system for your application
- Preparation of flow chambers and pump setups, i.e.:
- Guidelines on cell numbers and cultivation periods
- Seeding cells into channel slides
- How to avoid introducing air bubbles into your system
- Physical values that impact cell culture, i.e.:
- Flow chamber geometry and shear stress
- Laminar flow versus turbulent flow
Helga Wagner, ibidi GmbH
Helga Wagner studied Biology at the University of Regensburg. Since 2008 she is working in Research & Development of custom specific slides for functional cell based assays at ibidi GmbH. Her focus is on the design and implementation of tube formation assays and flow assays.