Oxygenation map for an SK-N-AS cell spheroid (size ~500 µm) maintained in 8% O2 atmosphere
A variety of different cell and tissue models are currently being used by researchers to investigate, for example, cancer development, tissue repair, and metabolic responses. However, many of these models have inadequate control of oxygenation conditions and physiological relevance. Optical O2 sensing technology, based on phosphorescence quenching, allows for the precise monitoring of O2. A quantitative measurement of the O2 concentration can be conducted in the gas or liquid phase, macroscopically or microscopically, using point measurements or high-resolution imaging in a non-invasive and flexible manner. This webinar will discuss the weaknesses of common hypoxia workstations, and then describe the available O2 sensing and imaging systems that can and should be integrated in experimental procedures for reliable in-situ control of O2, particularly at the cellular level.
Dmitri B. Papkovsky, PhD, School of Biochemistry and Cell Biology, University College Cork, Ireland
Prof. Dmitri B. Papkovsky received his PhD in 1986. Since 1997 he has been working at the University College Cork, School of Biochemistry and Cell Biology. His main research interests are in phosphorescence-based O2 sensing and imaging and their applications in hypoxia research, physiological and metabolic studies with various cells and 3D tissue models. He is one of the founders and the CSO of Luxcel Biosciences, has 23 patent families and 100+ publications. Several new technologies and products developed by Prof. Papkovsky have been commercialized and are being used by many research groups and pharmaceutical companies.