FAQ: Adenovirus Transduction

What makes adenoviral vectors such suitable tools for gene expression experiments?

Due to the broad host tropism of adenoviruses, they are ideal vectors for transferring genes into the majority of mammalian cells. Adenoviruses are episomal vectors that do not integrate into the host genome. They allow for the transient, high-level expression of recombinant proteins, and they also mediate very efficient gene knockdowns. The ability of the adenoviral vectors to be purified to high titers (up to 1013 virus particles per ml) makes them ideal tools for in vivo applications. Also, as adenoviruses possess the important attribute of stability in the bloodstream, they can be employed for gene delivery, following intravenous administration.

How does the ibiBoost Adeno Adenovirus Transduction Enhancer work?

ibiBoost Adeno is based on an adenovirus binding peptide. When added to adenovirus particles, ibiBoost changes the adenovirus surface in a way that the uptake of adenovirus is improved for all permissive cell types, and it allows transduction of cell types previously not accessible to adenovirus transduction. Unlike chemical transduction enhancers, ibiBoost has no toxic effects. The use of ibiBoost is especially recommended if high ”Multiplicities of Infection“ (MOI), exceeding 500, have to be applied to a particular cell type.