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Experimental Examples

Low Resolution Phase Contrast Imaging of a Tube Formation Assay
with Endothelial Cells

  • Endothelial cells
  • Tube formation assay on Matrigel™
  • Time between frames: 10 minutes
  • Total duration: 24 h
  • Objective lens: 4x

High Resolution Imaging of Molecular Processes in Chemotactical Polarized Cells Using LifeAct-TagGFP2

  • Mouse primary dendritic cell
  • Chemotaxis towards CCl 19
  • Migration in Collagen I gel matrix
  • Time between frames: 10 seconds
  • Total duration: 4 min
  • Objective lens: 100x

High Resolution Life Cell Imaging of Contracting Cardiomyocytes

  • Human iPSC-derived cardiomyocytes
  • Fuse-It-mRNA vesicles filled with LifeAct®-TagGFP2-mRNA
  • Membrane fusion using Fuse-It-mRNA
  • Contraction rate: 70 beats per minute
  • Objective lens: 100x