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The Principle of the µ-Slide Angiogenesis

The ibidi tube formation assay using the µ-Slide Angiogenesis was primarily developed to simplify the assay of endothelial cells on Matrigel™. Using a geometrical trick called “well-in-a-well“, the gel forms a flat surface (no meniscus), which provides all the cells in one optical plane. Additionally, the amount of Matrigel needed is reduced to 10 μl per well. After the gel is filled in and given time to solidify, the cells can be seeded on top and tube formation can be analyzed.

ibidi provides two different types of plates that contain this special geometry for angiogenesis assays (e.g., the tube formation assay). The first is the μ-Slide Angiogenesis, with 15 wells, for low throughput applications. The second, for larger screening applications, is ibidi’s μ-Plate Angiogenesis 96 Well.

ibidi’s „Well-in-a-Well“ Compared to a Standard Well

µ-Plate Angiogenesis
96 Well

  1. Planar air-liquid interface:
    good phase contrast all over the observation area
  2. Planar gel surface:
    all cells are in one optical plane

Volume of Matrigel: 10 µl

Standard well

  1. Meniscus on air-liquid interface:
    poor phase contrast in most of the observation area
  2. Mensicus on the gel surface: not possible to focus on all cells simultaneously

Volume of Matrigel: 100 µl

For more detailed information:

For practical details please watch the ibidi movie Performing an Angiogenesis Assay (MV 15).

In our Application Notes

you will find detailed information on how to setup and optimize tube formation experiments, data analysis, and interpretation.