µ-Plate Angiogenesis 96 well

A µ-Plate 96 well used to investigate angiogenesis in high throughput tube formation assays

Fully SBS (Society for Biomolecular Screening) and robotics compatible screening plate
Brilliant visualization without meniscus formation, and with all cells in one focal plane
Cost-effective high throughput experiments, requiring only 10 µl of gel per well
Also available in a version for low resolution microscopy

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Cat. No.	Description	Pcs./Box
89646	µ-Plate Angiogenesis 96 well, ibiTreat, tissue culture treated, sterile	15

Instructions

Instructions µ-Plate Angiogenesis 96 well (PDF)
Instructions µ-Plate Angiogenesis 96 well LR – coming soon

Applications:

Tube formation assays
Sprouting assays
3D cell culture
Immunofluorescence staining
Cultivation and microscopy of both living and fixed cells

Technical Features:

Standard SBS format
Two types of plates available:
µ-Plate Angiogenesis 96 well with ibidi Standard Bottom (180 µm) for high resolution microscopy
µ-Plate Angiogenesis 96 well LR with thick plate-like bottom (1 mm) for low resolution (LR) microscopy only

Specifications:

Number of wells	96
Length / Width	127.7 / 85.5 mm
Heigh with / without lid	16.6 / 14.4 mm
Well-to-well distance	9.0 mm
Volume inner wells	10 µl
Ø Inner wells	4 mm
Volume upper wells	70 µl
Ø Upper wells	5 mm
Growth area per inner well	0.125 cm²
Coating area per inner well	0.23 cm²
Well clearance	1.0 mm
Bottom 89646: ibidi Standard Bottom (180 µm) Bottom 89661: Thick plate-like (1 mm)

Angiogenesis Assays in vitro

Cells on gel matrices can be used to monitor their ability to form new vascular structures. Tube formation assays are carried out by seeding single cells onto the gel matrix and observing characteristic patterns. To carry out sprouting assays, either spheroids or pieces of tissue, e.g., from the aorta, are placed onto the gel matrix. Both these types of assays require that the underlying gel layer be of uniform thickness.

µ-Plate Angiogenesis 96 well vs. Standard 96 well Plate

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µ-Plate Angiogenesis 96 well

Planar air-liquid interface:
good phase contrast all over the observation area
Planar gel surface:
all cells are in one optical plane

Volume of Matrigel: 10 µl

Standard well

Meniscus on air-liquid interface:
poor phase contrast in most of the observation area
Mensicus on the gel surface: not possible to focus on all cells simultaneously

Volume of Matrigel: 100 µl

Only Four Steps from Sample Preparation to Image Analysis

Tube Formation Image Analysis is a crucial step in angiogenesis assays. Wimasis, the certified partner of ibidi for quantitative image analysis, developed an analysis solution to quantitatively evaluate the generation of cellular networks, which is called Tube Formation Image Analysis - WimTube.

First, use the µ-Plate Angiogenesis 96 well to perform your tube formation experiments and acquire microscopy images. Then, simply upload the images to the image analysis platform and receive your results via E-mail.
Use our Free Trial to test this new Image Analysis solution.

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