• µ-Plate Angiogenesis 96 well
  • µ-Plate Angiogenesis 96 well

µ-Plate Angiogenesis 96 well

A µ-Plate 96 well used to investigate angiogenesis in high throughput tube formation assays

  • Fully SBS (Society for Biomolecular Screening) and robotics compatible screening plate
  • Brilliant visualization without meniscus formation, and with all cells in one focal plane
  • Cost-effective high throughput experiments, requiring only 10 µl of gel per well

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Cat. No. Description Pcs./Box
89646 µ-Plate Angiogenesis 96 well, ibiTreat, tissue culture treated, sterile 15

Applications:


Technical Features:

 

Specifications:

Number of wells 96
Length / Width 127.7 / 85.5 mm
Heigh with / without lid 16.6 / 14.4 mm
Well-to-well distance 9.0 mm
Volume inner wells 10 µl
Ø Inner wells 4 mm
Volume upper wells 70 µl
Ø Upper wells 5 mm
Growth area per inner well 0.125 cm²
Coating area per inner well 0.23 cm²
Well clearance 1.0 mm
Bottom: ibidi Standard Bottom

Angiogenesis Assays in vitro

 

 

Cells on gel matrices can be used to monitor their ability to form new vascular structures. Tube formation assays  are carried out by seeding single cells onto the gel matrix and observing characteristic patterns. To carry out sprouting assays, either spheroids or pieces of tissue, e.g., from the aorta, are placed onto the gel matrix. Both these types of assays require that the underlying gel layer be of uniform thickness.


µ-Plate Angiogenesis 96 well vs. Standard 96 well Plate

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µ-Plate Angiogenesis 96 well

  1. Planar air-liquid interface:
    good phase contrast all over the observation area
  2. Planar gel surface:
    all cells are in one optical plane


Volume of Matrigel: 10 µl

 

Standard well

  1. Meniscus on air-liquid interface:
    poor phase contrast in most of the observation area
  2. Mensicus on the gel surface: not possible to focus on all cells simultaneously

Volume of Matrigel: 100 µl


Only Four Steps from Sample Preparation to Image Analysis

Tube Formation Image Analysis is a crucial step in angiogenesis assays. Wimasis, the certified partner of ibidi for quantitative image analysis, developed an analysis solution to quantitatively evaluate the generation of cellular networks, which is called Tube Formation Image Analysis - WimTube.

First, use the µ-Plate Angiogenesis 96 well to perform your tube formation experiments and acquire microscopy images. Then, simply upload the images to the image analysis platform and receive your results via E-mail.
Use our Free Trial to test this new Image Analysis solution.


in cooperation with


Sam Noppen, Rega Institute for Medical Research, Leuven, Belgium

"I used the ibidi µ-Plate Angiogenesis 96 well for bioluminescence measurements. The plate performed exceptionally well for this specific application, as it reduced my sample volume drastically and the optical quality was just superb."

Sam Noppen
Rega Institute for Medical Research, KU Leuven
Leuven
Belgium

http://www.kuleuven.be/rega/cmt/

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