µ-Slide Angiogenesis

A µ-Slide used to investigate angiogenesis in tube formation assays. Also perfect for 3D cell culture and immunofluorescence staining.

Complete solution for tube formation experiments, requiring only a few steps from sample preparation to image analysis
Brilliant visualization without meniscus formation, and with all cells in one focal plane
Can be used with a broad range of gels, e.g. Matrigel™, collagen, and agarose
Cost-effective experiments, requiring only 10 µl of gel per well

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Cat. No.	Description	Pcs./Box
81506	µ-Slide Angiogenesis, ibiTreat, tissue culture treated, sterile	15
81501	µ-Slide Angiogenesis, hydrophobic, uncoated, sterile	15
81531	µ-Slide Angiogenesis, Microdissection, PEN-membrane, sterile	15

Applications:

Tube formation assays
Sprouting assays
3D cell culture
Immunofluorescence staining
Cultivation and microscopy of both living and fixed cells

Technical Features:

Standard slide format
Closely fitting lid to reduce evaporation
4 mm well within a 5 mm well
Homogeneous 0.8 mm thick gel layer*
Homogeneous cell growth
Compatible with staining and fixation
Excellent optical properties for microscopy
Compatible with multi-channel pipettes
Made of biocompatible plastic material - no glue, no leaking
Suitable for use with various types of gels, e.g. Matrigel™, collagen, and agarose*
Also available in a 96 well format: µ-Plate Angiogenesis 96 well

*The gel matrix is not part of the product.

Specifications:

Number of wells	15
Volume inner well	10 µl
Ø inner well	4 mm
Volume upper well	50 µl
Ø upper well	5 mm
Growth area per inner well	0.125 cm²
Coating area per inner well	0.23 cm²
Bottom: ibidi Standard Bottom

Define and print your experimental setup

Fluorescence microscopy of one single strand composed of HUVEC cells. F-actin cytoskeleton stained green, and cell nuclei blue.

Angiogenesis Assays in vitro

Cells on gel matrices can be used to monitor their ability to form new vascular structures. Tube formation assays are carried out by seeding single cells onto the gel matrix and observing characteristic patterns. To carry out sprouting assays, either spheroids or pieces of tissue, e.g., from the aorta, are placed onto the gel matrix. Both these types of assays require that the underlying gel layer be of uniform thickness.

“Well-in-a-well” feature avoids meniscus formation

The μ-Slide Angiogenesis requires only 10 µl of gel per well. It is suitable for use with all common gel matrices, such as Matrigel™, collagen gels and hyaluronic acid gels. Another advantage of the µ-Slide Angiogenesis is that assays benefit from having a gel matrix of uniform thickness. Furthermore, not only does this slide provide reproducible cell culture conditions, it also ensures all cells are in one optical plane.

μ-Slide Angiogenesis versus Standard Well

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µ-Slide Angiogenesis

Planar air-liquid interface:
good phase contrast all over the observation area
Planar gel surface:
all cells are in one optical plane

Volume of Matrigel: 10 µl

Standard well

Meniscus on air-liquid interface:
poor phase contrast in most of the observation area
Mensicus on the gel surface: not possible to focus on all cells simultaneously

Volume of Matrigel: 100 µl

Only Four Steps from Sample Preparation to Image Analysis

Tube Formation Image Analysis is a crucial step in angiogenesis assays. Wimasis, the certified partner of ibidi for quantitative image analysis, developed an analysis solution to quantitatively evaluate the generation of cellular networks, which is called Tube Formation Image Analysis - WimTube.

First, use the µ-Slide Angiogenesis to perform your tube formation experiments and acquire microscopy images. Then, simply upload the images to the image analysis platform and receive your results via E-mail.
Use our Free Trial to test this new Image Analysis solution.

Example Data

Images from a Human Umbilical Vein Endothelial Cells (HUVECs) tube formation experiment on growth factor reduced matrigel were analyzed by Wimasis (WimTube low magnification). The data from 8 separate experiments were summarized into one data point, using standard deviation as the error bar. The graph shows the tube length per standard area (i.e., 1 mm²) at 1, 4, 6 and 24 hours.

in cooperation with

The µ-Slide Angiogenesis Can Be Used in a Wide Range of Applications

	Normal use - for tube formation assays Tube formation assays are carried out by seeding single cells on top of a 10 µl gel layer and observing characteristic patterns.
	Lower volumes of gel - for focusing cells When used with less than 10 µl of gel the µ-Slide Angiogenesis can be used to cultivate small numbers of cells on a soft gel surface.
	Cells embedded in gel matrix – 3D cell culture The „well in a well“ feature of the μ-Slide Angiogenesis also supports microscopy of cells embedded in gel matrices. 3D cell cultures mimic in vivo conditions of, e.g., cancer cells and hepatocytes. The combination of the gel matrix with the medium reservoir above it allows for fast and easy medium exchange by diffusion.
	Without any gel matrix – low volume microscopy chamber The µ-Slide Angiogenesis can also be used without any gel. In this fashion it can be used as a 15 fold sample chamber for, e.g., coating experiments.
	Also applicable for IF-based coating tests