A µ-Slide used to investigate angiogenesis in tube formation assays. Also perfect for 3D cell culture and immunofluorescence staining.
- Complete solution for tube formation experiments, requiring only a few steps from sample preparation to image analysis
- Brilliant visualization without meniscus formation, and with all cells in one focal plane
- Can be used with a broad range of gels, e.g. Matrigel™, collagen, and agarose
- Cost-effective experiments, requiring only 10 µl of gel per well
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|81506||µ-Slide Angiogenesis, ibiTreat: #1.5 polymer coverslip, tissue culture treated, sterilized||15|
|81501||µ-Slide Angiogenesis, Uncoated: #1.5 polymer coverslip, hydrophobic, sterilized||15|
|81531||µ-Slide Angiogenesis, Microdissection: PEN membrane, sterilized||15|
Define and print your experimental setup
Fluorescence microscopy of one single strand composed of HUVEC cells. F-actin cytoskeleton stained green, and cell nuclei blue.
Cells on gel matrices can be used to monitor their ability to form new vascular structures. Tube formation assays are carried out by seeding single cells onto the gel matrix and observing characteristic patterns. To carry out sprouting assays, either spheroids or pieces of tissue, e.g., from the aorta, are placed onto the gel matrix. Both these types of assays require that the underlying gel layer be of uniform thickness.
μ-Slide Angiogenesis versus Standard Well
Only Four Steps from Sample Preparation to Image Analysis
Images from a Human Umbilical Vein Endothelial Cells (HUVECs) tube formation experiment on growth factor reduced matrigel were analyzed by Wimasis (WimTube low magnification). The data from 8 separate experiments were summarized into one data point, using standard deviation as the error bar. The graph shows the tube length per standard area (i.e., 1 mm²) at 1, 4, 6 and 24 hours.
The µ-Slide Angiogenesis Can Be Used in a Wide Range of Applications
Kaela Varberg, Indiana University, School of Medicine, Indianapolis, USA
“The µ-Slides Angiogenesis created by ibidi have significantly increased the efficiency and accuracy of the angiogenesis assays in our lab. Using the ibidi slides not only reduces the amount of time spent capturing images, but it also reduces the time it takes to quantify the data since the structures are in the same focal plane. Another added bonus is the reduced amount of matrigel required for each experiment which saves money!”
School of Medicine
Dr. Esther G.L. Koh, National University of Singapore, Singapore
“ibidi made it much simpler for me to prepare cells for confocal microscopy and live-cell timelapse microscopy. Cells that attached poorly to glass grew better on ibidi µ-Slides/Dishes. The angiogenesis slide allowed me to minimise the amount of cells and reagents required for an experiment.”
Dr. Esther G.L. Koh
Head, Advanced Imaging Laboratory
Life Sciences Institute Immunology Programme
National University of Singapore
Centre for Life Sciences
Prof. Dr. Stefan Zahler, University of Munich, Germany
„Using the „angiogenesis slide“ from ibidi is the only possibility that I know for achieving a consistently good optical quality in the tube formation assay, and saving Matrigel at the same time.
In comparison to other systems, the heating stage from ibidi shows superior thermal stability and enables you to work with high humidity (>80%). This is not possible with other systems but is indispensable for long-term studies.“
Prof. Dr. Stefan Zahler
Munich Center for System-Based Drug Research