• µ-Slide 8 well
  • µ-Slide 8 well

µ-Slide 8 well

An open µ-Slide (chambered coverslip) with 8 wells for use in immunofluorescence and high-end microscopy

  • All-in-one chamber slide reduces the number of experimental steps in immunofluorescence protocols
  • Observation of the sample through the coverslip-like bottom using high resolution microscopy
  • Fast and easy sample preparation: No coverslips, no leakage
  • Cost-effective experiments using small numbers of cells and low volumes of reagents

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Cat. No. Description Pcs./Box
80826 µ-Slide 8 well, ibiTreat, tissue culture treated, sterile 15
80822 µ-Slide 8 well, Collagen IV, sterile 15
80823 µ-Slide 8 well, Fibronectin, sterile (available only on request) 15
80824 µ-Slide 8 well, Poly-L-Lysine, sterile 15
80825 µ-Slide 8 well, Poly-D-Lysine, sterile (available only on request) 15
80821 µ-Slide 8 well, hydrophobic, uncoated, sterile 15

Applications:


Technical Features:

  • Open µ-Slide (chambered coverslip) with 8 independent wells
  • Excellent optical quality imaging chamber for high-end microscopy
  • Compatible with staining and fixation
  • ibiTreat surface for optimal cell adhesion
  • Biocompatible plastic material – no glue, no leaking
  • Also available in an adhesive version without a bottom: sticky-Slide 8 well
  • Also available with a glass bottom
 

Specifications:

Number of wells 8
Dimensions of wells (w x l x h) in mm 9.4 x 10.7 x 6.8
Volume per well 300 µl
Total height with lid 8 mm
Growth area per well 1.0 cm²
Coating area per well 2.20 cm²
Bottom: ibidi Standard Bottom


Define and print your experimental setup



The ibidi µ-Slide 8 well allows standard immunofluorescence protocols to be employed without the use of coverslips in an all-in-one chamber. All steps (i.e., cell cultivation, fixation, staining and imaging) are carried out in the classic open well geometry. After staining, the sample can be observed through the coverslip-like bottom using high resolution microscopy. The closely fitting lid prevents evaporation and allows for long-term assays.


 

Closely fitting lid
Suitable for high resolution microscopy

Other Possible Applications:

Can be used with Culture-Inserts To test different compound concentrations, e.g., in stem cell development  


Owen McCarty, PhD, Oregon Health & Science University, Portland, USA

“The ibidi platforms have opened new possibilities for our group to utilize small volumes of blood to characterize the cell biology of platelets and white blood cells. Moreover, we have greatly benefited from collaborating with the technical representatives at ibidi to discuss ways to multiplex the ibidi platforms to address our experimental questions.”

Owen McCarty, PhD
Associate Professor
Department of Biomedical Engineering
Oregon Health & Science University
Portland, Oregon
USA

http://www.ohsu.edu/xd/education/schools/school-of-medicine/departments/basic-science-departments/biomedical-engineering/


Joanne Marrison, University of York, UK

"I plated and transfected cells on the ibidi µ-Slide 8 well alongside the regular chambers we normally use and they were very good. In fact, I particularly liked the lids which seem more secure than our regular 8-well chambers and the fact that they are 'slide' sized with space for writing a label. Another bonus was the individual packaging which gives me greater confidence on sterility particularly when not all the chambers in a pack of 8 are used at once. So all in all great."

Joanne Marrison
Imaging and Cytometry Laboratory (B/K051)
Bioscience Technology Facility, Department of Biology
University of York, York
United Kingdom

www.york.ac.uk/biology/tf


Prof. Dr. Markus Sauer, University of Wuerzburg, Germany

„ibidi slides are compelling!
We tested the open μ-Slides with 8 wells for live-cell super-resolution imaging by dSTORM with SNAP-tags. The quality of the super-resolved images was impressive.“

Prof. Dr. Markus Sauer
Biotechnology & Biophysics
Julius-Maximilians-University Wuerzburg
Germany

http://www.super-resolution.biozentrum.uni-wuerzburg.de/research_topics/super_resolution_imaging/

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