• µ-Slide 2 x 9 well
  • µ-Slide 2 x 9 well

µ-Slide 2 x 9 well

A µ-Slide (chambered coverslip) suitable for co-cultivation assays in combination with microscopy

  • Cells share soluble factors but grow separately
  • Possesses low evaporation properties and is thus suitable for long-term assays
  • Has excellent optical quality and is therefore suitable for fluorescence microscopy

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Cat. No. Description Pcs./Box
81806 µ-Slide 2 x 9 well, ibiTreat, tissue culture treated, sterile 15
81802 µ-Slide 2 x 9 well, Collagen IV, sterile 15
81803 µ-Slide 2 x 9 well, Fibronectin, sterile (available only on request) 15
81804 µ-Slide 2 x 9 well, Poly-L-Lysine, sterile 15
81805 µ-Slide 2 x 9 well, Poly-D-Lysine, sterile (available only on request) 15
81801 µ-Slide 2 x 9 well, hydrophobic, uncoated, sterile 15

Applications:

  • Co-cultivation of different cell lines or primary cells
  • Mesenchymal-epithelial interactions
  • Paracrine interaction of different cell populations in vitro
  • Cell or spheroid culture in gel matrices


Technical Features:

  • Two major wells each with 9 minor wells
  • Cells share soluble factors but grow separately
 

Specifications:

Number of major wells 2
Volume per major well 600 µl
Dimensions of major wells
(w x l x h) in mm
21.5 x 23.6 x 6.8
Number of minor wells 2 x 9
Volume of each minor well 70 µl
Dimensions of minor wells
(w x l x h) in mm
6.1 x 6.8 x 1.3
Growth area per minor well 0.4 cm2
Coating area per minor well 0.55 cm2
Bottom: ibidi Standard Bottom


Define and print your experimental setup

Seeding Cells

Step 1:

Seed 60 µl of recipient cells in the
inner reservoir.

Step 2:

Seed 60 µl of the feeder cells in the
outer reservoirs.
Await cell attachment; aspirate all
unattached cells and medium.

Step 3:

Overlay each major well with 600 µl cell free medium.


Detailed Cross Section with Indicated Conditioned Medium


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