µ-Slide 2 well

An open µ-Slide (chambered coverslip) with 2 wells for use in immunofluorescence and high-end microscopy

All-in-one chamber slide used for cell culture and microscopy
Observation of the sample through the coverslip-like bottom using high resolution microscopy
Fast and easy immunofluorescence, with no coverslips and no leakage
Cost-effective experiments using small numbers of cells and low volumes of reagents

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Cat. No.	Description	Pcs./Box
80286	µ-Slide 2 well, ibiTreat, tissue culture treated, sterile	15
80281	µ-Slide 2 well, hydrophobic, uncoated, sterile	15
80285	µ-Slide 2 well, Poly-D-Lysine, sterile (available only on request)	15
80284	µ-Slide 2 well, Poly-L-Lysine, sterile	15
80282	µ-Slide 2 well, Collagen IV, sterile	15
80283	µ-Slide 2 well, Fibronectin, sterile (available only on request)	15

Applications:

Cultivation and microscopy of cell cultures
Transfection assays
Fluorescence microscopy of both living and fixed cells
Live cell imaging over extended time periods

Technical Features:

Open µ-Slide with 2 independent wells
Intermediate plate in each well to avoid meniscus formation
Easy filling, without air bubbles, through the lateral slot
Excellent optical quality for high-end microscopy
Compatible with staining and fixation
ibiTreat surface for optimal cell adhesion
Made of biocompatible plastic material – no glue and no leaking

Specifications:

Number of wells	2
Dimensions of wells (w x l x h) in mm	21.2 x 23.3 x 9.3
Volume per well	700 µl
Total height with lid	10.8 mm
Growth area per well	2.2 cm²
Coating area per well	11.4 cm²
Bottom:	ibidi Standard Bottom

Phase contrast microscopy is the most commonly used, transmitted light technique in cell culture. When working with phase contrast microscopy, it is crucial to have the two phase rings adjusted to each other. In open wells, the meniscus at the air-water-interphase works like a lens that refracts the beam path. This miscalibrates the phase rings, leading to poor contrast in the microscopic image.

Working with the µ-Slide 2 well Ph+ diminishes the meniscus, so that the whole optical system is aligned, no matter which position of the well is being imaged.

µ-Slide 2 well

µ-Slide 2 well^Ph+

Top View

The µ-Slide 2 well^Ph+ (Phase Contrast +) is designed for excellent optical quality, especially for cell culture when normal phase contrast microscopy is being used. Opposed to the classic µ-Slide 2 well, the Ph+ version provides a special intermediate plate in the center of the well. This plate flattens the meniscus that disturbs the phase contrast effect in normal open wells. Openings near the corners provide access to the wells for the easy filling and aspirating of liquids.

This innovative technique supports meniscus-free phase contrast microscopy in a very convenient manner.

Cross Section Through a Well of the µ-Slide 2 well^Ph+ with a Transmitted Light Beam Path

The illustration on the left shows the perturbing effect of a meniscus. Light is refracted on the air-water-interface, leading to poor contrast in microscopy. Only the small center part exhibits satisfying phase contrast.

Working with the µ-Slide 2 well^Ph+ diminishes the meniscus and increases the area of nicely contrasted cells. This nice contrast is due to the parallel beam path that is created by the plate.