• µ-Slide 2 well
  • µ-Slide 2 well

µ-Slide 2 well

An open µ-Slide (chambered coverslip) with 2 wells for use in immunofluorescence and high-end microscopy

  • All-in-one chamber slide used for cell culture and microscopy
  • Observation of the sample through the coverslip-like bottom using high resolution microscopy
  • Fast and easy immunofluorescence, with no coverslips and no leakage
  • Cost-effective experiments using small numbers of cells and low volumes of reagents

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Cat. No. Description Pcs./Box
80286 µ-Slide 2 well, ibiTreat, tissue culture treated, sterile 15
80281 µ-Slide 2 well, hydrophobic, uncoated, sterile 15
80285 µ-Slide 2 well, Poly-D-Lysine, sterile (available only on request) 15
80284 µ-Slide 2 well, Poly-L-Lysine, sterile 15
80282 µ-Slide 2 well, Collagen IV, sterile 15
80283 µ-Slide 2 well, Fibronectin, sterile (available only on request) 15

Applications:

  • Cultivation and microscopy of cell cultures
  • Transfection assays
  • Fluorescence microscopy of both living and fixed cells
  • Live cell imaging over extended time periods
  • Suitable for DIC, when used with a DIC lid


Technical Features:

  • Open µ-Slide with 2 independent wells
  • Excellent optical quality for high-end microscopy
  • Compatible with staining and fixation
  • ibiTreat surface for optimal cell adhesion
  • Made of biocompatible plastic material – no glue and no leaking
  • Also available with a glass bottom
 

Specifications:

Number of wells 2
Dimensions of wells
(w x l x h) in mm
21.2 x 23.3 x 9.3
Volume per well 1500 µl
Total height with lid 10.8 mm
Growth area per well 4.8 cm²
Coating area per well 7.5 cm²
Bottom: ibidi Standard Bottom


Phase contrast microscopy is the most commonly used, transmitted light technique in cell culture. When working with phase contrast microscopy, it is crucial to have the two phase rings adjusted to each other. In open wells, the meniscus at the air-water-interphase works like a lens that refracts the beam path. This miscalibrates the phase rings, leading to poor contrast in the microscopic image.

Working with the µ-Slide 2 well Ph+ diminishes the meniscus, so that the whole optical system is aligned, no matter which position of the well is being imaged.

 


µ-Slide 2 well

µ-Slide 2 well Ph+

Top View

 

 


The µ-Slide 2 well Ph+ (Phase Contrast +) is designed for excellent optical quality, especially for cell culture when normal phase contrast microscopy is being used. Opposed to the classic µ-Slide 2 well, the Ph+ version provides a special intermediate plate in the center of the well. This plate flattens the meniscus that disturbs the phase contrast effect in normal open wells. Openings near the corners provide access to the wells for the easy filling and aspirating of liquids.

This innovative technique supports meniscus-free phase contrast microscopy in a very convenient manner. 


Cross Section Through a Well of the µ-Slide 2 well Ph+ with a Transmitted Light Beam Path

 




The illustration on the left shows the perturbing effect of a meniscus. Light is refracted on the air-water-interface, leading to poor contrast in microscopy. Only the small center part exhibits satisfying phase contrast.

Working with the µ-Slide 2 well Ph+ diminishes the meniscus and increases the area of nicely contrasted cells. This nice contrast is due to the parallel beam path that is created by the plate.


Owen McCarty, PhD, Oregon Health & Science University, Portland, USA

“The ibidi platforms have opened new possibilities for our group to utilize small volumes of blood to characterize the cell biology of platelets and white blood cells. Moreover, we have greatly benefited from collaborating with the technical representatives at ibidi to discuss ways to multiplex the ibidi platforms to address our experimental questions.”

Owen McCarty, PhD
Associate Professor
Department of Biomedical Engineering
Oregon Health & Science University
Portland, Oregon
USA

http://www.ohsu.edu/xd/education/schools/school-of-medicine/departments/basic-science-departments/biomedical-engineering/

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