• µ-Dish<sup> 50mm, low</sup>
  • µ-Dish<sup> 50mm, low</sup>

µ-Dish 50mm, low

A 50 mm imaging dish with an ibidi Standard Bottom and low walls, used for high end microscopy and micromanipulation

  • Largest growth area and volume of all ibidi products
  • Large access for micromanipulation
  • Unique ibidi Standard Bottom combining
    • Excellent cell culture conditions; and
    • Supreme optical quality for high resolution microscopy
  • Ideal cell growth conditions provided by the ibiTreat surface
  • Lid with lock position, which minimizes evaporation

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Cat. No. Description Pcs./Box
81136 µ-Dish 50 mm, low, ibiTreat: Ø 50 mm, low wall (3 ml volume), #1.5 polymer coverslip, tissue culture treated, sterilized 30
81131 µ-Dish 50 mm, low, hydrophobic: Ø 50 mm, low wall (3 ml volume), #1.5 polymer coverslip, hydrophobic, sterilized 30

Applications

  • Cell culture and high resolution fluorescence microscopy
  • Cultivation of a maximum number of cells
  • Micromanipulation of single cells
  • Fluorescence microscopy of both living and fixed cells


Technical Features

  • Standard format dish with a 50 mm diameter
  • Made of biocompatible material using no glues, consequently no cells are harmed
  • Rim for easy opening
  • No autofluorescence
  • Compatible with all staining and fixation solvents


Low walls
- Large access for micromanipulation

 

Specifications:

Ø µ-Dish 50 mm
Volume 3 ml
Growth area 7.0 cm2
Coating area using 700 µl 7.9 cm2
Ø observation area 30 mm
Height with / without lid 12 / 9 mm
Bottom: ibidi Standard Bottom  



Immunofluorescence of mouse cells
Blue: nucleus (DAPI)
Green: tubulin (Alexa488)

Lid with Lock Position

The special lock feature in all ibidi µ-Dishes enables the user to minimize evaporation. The lock position provides excellent conditions for long term studies in non humidified environments. Gas exchange (carbon dioxide, oxygen) during cell culture is maintained thanks to the gas permeable plastic material of the dish.

TIP: Use the locking feature only if minimal evaporation is
required, i.e., outside incubators, non humidified microscopy stages, etc.

 

click here to enlarge

ibidi µ-Dish Family: Versions and Variations

µ-Dish 35 mm, high       
µ-Dish 35 mm, low µ-Dish 50 mm, low

High walls:
Used for all standard  applications

Low walls:
Provide greater cell access (useful for micromanipulation)

                                         

ibidi Standard Bottom vs. ibidi Glass Bottom


ibidi Standard Bottom

ibidi Glass Bottom

Optical properties
Refractive index (nD 589 nm) 1.52 1.52
Abbe number 56 55
Thickness #1.5 (180 µm) #1.5H (170 µm)
Material Microscopy plastic D 263M Schott borosilicate glass
Autofluorescence Low Low
Transmission Very high (even ultraviolet) High (ultraviolet restrictions)
Birefringence (DIC) Low (DIC compatible*) Low (DIC compatible*)
Other aspects
Surface modifications ibiTreat – tissue culture treated
Uncoated – hydrophobic
Only pure glass
Protein coatings Possible Possible
Gas permeable Yes No
Material flexibility High Low
Breakable No Yes
Recommended for All kinds of fluorescence microscopy TIRF and single photon

* Special glass DIC Lids are available separately


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