µ-Dish^{50mm, low}

A 50 mm imaging dish with an ibidi Standard Bottom and low walls, used for high end microscopy and micromanipulation

Largest growth area and volume of all ibidi products
Large access for micromanipulation
Unique ibidi Standard Bottom combining
- Excellent cell culture conditions; and
- Supreme optical quality for high resolution microscopy
Ideal cell growth conditions provided by the ibiTreat surface
Lid with lock position, which minimizes evaporation

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Cat. No.	Description	Pcs./Box
81136	µ-Dish 50 mm, low ibiTreat, tissue culture treated, sterile, Ø 50 mm, 3 ml volume	30
81131	µ-Dish 50 mm, low hydrophobic, uncoated, sterile, Ø 50 mm, 3 ml volume	30

Applications

Cell culture and high resolution fluorescence microscopy
Cultivation of a maximum number of cells
Micromanipulation of single cells
Fluorescence microscopy of both living and fixed cells

Technical Features

Standard format dish with a 50 mm diameter
Made of biocompatible material using no glues, consequently no cells are harmed
Rim for easy opening
No autofluorescence
Compatible with all staining and fixation solvents

Low walls - Large access for micromanipulation

Specifications:

Ø µ-Dish	50 mm
Volume	3 ml
Growth area	7.0 cm²
Coating area using 700 µl	7.9 cm²
Ø observation area	30 mm
Height with / without lid	12 / 9 mm
Bottom: ibidi Standard Bottom

Immunofluorescence of mouse cells
Blue: nucleus (DAPI)
Green: tubulin (Alexa488)

Lid with Lock Position

The special lock feature in all ibidi µ-Dishes enables the user to minimize evaporation. The lock position provides excellent conditions for long term studies in non humidified environments. Gas exchange (carbon dioxide, oxygen) during cell culture is maintained thanks to the gas permeable plastic material of the dish.

TIP: Use the locking feature only if minimal evaporation is
required, i.e., outside incubators, non humidified microscopy stages, etc.

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ibidi µ-Dish Family: Versions and Variations

µ-Dish^{35 mm, high}	µ-Dish^{35 mm, low}	µ-Dish^{50 mm, low}


High walls: Used for all standard applications	Low walls: Provide greater cell access (useful for micromanipulation)

ibidi Standard Bottom vs. ibidi Glass Bottom

	ibidi Standard Bottom	ibidi Glass Bottom
Optical properties
Refractive index (n_D 589 nm)	1.52	1.52
Abbe number	56	55
Thickness	#1.5 (180 µm)	#1.5 (170 µm)
Material	Microscopy plastic	D 263M Schott borosilicate glass
Autofluorescence	Low	Low
Transmission	Very high (even ultraviolet)	High (ultraviolet restrictions)
Birefringence (DIC)	Low (DIC compatible*)	Low (DIC compatible*)
Other aspects
Surface modifications	ibiTreat – tissue culture treated Uncoated – hydrophobic	Only pure glass
Protein coatings	Possible	Possible
Gas permeable	Yes	No
Material flexibility	High	Low
Breakable	No	Yes
Recommended for	All kinds of fluorescence microscopy	TIRF and single photon