• µ-Dish<sup> 35mm, low</sup>
  • µ-Dish<sup> 35mm, low</sup>

µ-Dish 35mm, low

A 35 mm imaging dish with an ibidi Standard Bottom and low walls, used in high end microscopy and micromanipulation

  • Large access for micromanipulation
  • Unique ibidi Standard Bottom combining
    • Excellent cell culture conditions; and
    • Supreme optical quality for high resolution microscopy
  • Ideal cell growth conditions provided by the ibiTreat surface
  • Lid with lock position, which minimizes evaporation
  • Suitable for DIC, when used with the special DIC Lid
  • Also available with a relocation grid

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Cat. No. Description Pcs./Box
80136 µ-Dish 35 mm, low, ibiTreat: Ø 35 mm, low wall (800 µl volume), #1.5 polymer coverslip, tissue culture treated, sterilized 60
80131 µ-Dish 35 mm, low, hydrophobic: Ø 35 mm, low wall (800 µl volume), #1.5 polymer coverslip, hydrophobic, sterilized 60

Applications

  • Cell manipulation and microinjection
  • Fluorescence microscopy of both living and fixed cells
  • Live cell imaging
  • Suitable for DIC, when used with the special DIC Lid


Technical Features

  • Standard format dish with a 35 mm diameter
  • Made of biocompatible material using no glues, consequently no cells are harmed
  • No autofluorescence
  • Compatible with all staining and fixation solvents


Low walls
- Large access for micromanipulation

 

Specifications:

Ø µ-Dish 35 mm
Volume 800 µl
Growth area 3.5 cm2
Coating area using 400 µl 4.1 cm2
Ø observation area 21 mm
Height with / without lid 9 / 7 mm
Bottom: ibidi Standard Bottom  



Lid with Lock Position

The special lock feature in all ibidi µ-Dishes enables the user to minimize evaporation. The lock position provides excellent conditions for long term studies in non humidified environments. Gas exchange (carbon dioxide, oxygen) during cell culture is maintained thanks to the gas permeable plastic material of the dish.

TIP: Use the locking feature only if minimal evaporation is
required, i.e., outside incubators, non humidified microscopy stages, etc.

 

click here to enlarge

ibidi µ-Dish Family: Versions and Variations

µ-Dish 35 mm, high       
µ-Dish 35 mm, low µ-Dish 50 mm, low

High walls:
Used for all standard  applications

Low walls:
Provide greater cell access (useful for micromanipulation)

                                         

ibidi Standard Bottom vs. ibidi Glass Bottom


ibidi Standard Bottom

ibidi Glass Bottom

Optical properties
Refractive index (nD 589 nm) 1.52 1.52
Abbe number 56 55
Thickness #1.5 (180 µm) #1.5H (170 µm)
Material Microscopy plastic D 263M Schott borosilicate glass
Autofluorescence Low Low
Transmission Very high (even ultraviolet) High (ultraviolet restrictions)
Birefringence (DIC) Low (DIC compatible*) Low (DIC compatible*)
Other aspects
Surface modifications ibiTreat – tissue culture treated
Uncoated – hydrophobic
Only pure glass
Protein coatings Possible Possible
Gas permeable Yes No
Material flexibility High Low
Breakable No Yes
Recommended for All kinds of fluorescence microscopy TIRF and single photon

* Special glass DIC Lids are available separately


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