• µ-Dish<sup> 35mm</sup> Grid-500
  • µ-Dish<sup> 35mm</sup> Grid-500

µ-Dish 35mm Grid-500

A 35 mm imaging dish with an ibidi Standard Bottom, low / high walls and an imprinted 500 µm relocation grid

  • Ideal for relocating cells or cell clusters
  • Unique ibidi Standard Bottom combining
    • Excellent cell culture conditions; and
    • Supreme optical quality for high resolution microscopy
  • Ideal cell growth conditions provided by the ibiTreat surface
  • Lid with lock position, which minimizes evaporation
  • Suitable for DIC, when used with the special DIC Lid

>> Order Your
Free Sample

For pricing and online ordering, please log in.

Cat. No. Description Pcs./Box
80156 µ-Dish 35 mm, low, ibiTreat, Grid-500: Ø 35 mm, low wall (800 µl volume), #1.5 polymer coverslip, tissue culture treated, grid repeat distance 500 µm, sterilized 60
80151 µ-Dish 35 mm, low, Uncoated, Grid-500: Ø 35 mm, low wall (800 µl volume), #1.5 polymer coverslip, hydrophobic, grid repeat distance 500 µm, sterilized 60
81166 µ-Dish 35 mm, high, ibiTreat, Grid-500: Ø 35 mm, high wall (2 ml volume), #1.5 polymer coverslip, tissue culture treated, grid repeat distance 500 µm, sterilized 60
81161 µ-Dish 35 mm, high, Uncoated, Grid-500: Ø 35 mm, high wall (2 ml volume), #1.5 polymer coverslip, hydrophobic, grid repeat distance 500 µm, sterilized 60

Applications

  • Relocating cells or cell clusters, e.g., transfected cells for clone picking
  • Counting events per defined area (e.g., for calculating transfection efficiency)
  • Providing a reference structure for cell movements
  • Treatment of distinct single cells, such as in microinjection
  • Following axon growth on a defined scale bar


Technical Features

  • Standard format dish with a 35 mm diameter
  • Grid with a 500 µm repeat distance
  • Lettered and numbered fields (A-U; 1-20)
  • Also available with a glass bottom: µ-Slide 35 mm, high glass bottom with Grid-50 or with Grid-500
  • Ideal cell growth conditions provided by the ibiTreat tissue culture treated surface
  • Made of biocompatible material using no glues, consequently no cells are harmed
  • Lid with lock position, which minimizes evaporation
 

Specifications:

Grid repeat distance 500 µm
Ø µ-Dish 35 mm
Volume high / low 2 / 0.8 ml
Growth area 3.5 cm2
Coating area using 400 µl 4.1 cm2
Ø observation area 21 mm
Height with lid high / low 14 / 9 mm
Bottom: ibidi Standard Bottom  



Layout Grid-500


Lid with Lock Position

The special lock feature in all ibidi µ-Dishes enables the user to minimize evaporation. The lock position provides excellent conditions for long term studies in non humidified environments. Gas exchange (carbon dioxide, oxygen) during cell culture is maintained thanks to the gas permeable plastic material of the dish.

TIP: Use the locking feature only if minimal evaporation is
required, i.e., outside incubators, non humidified microscopy stages, etc.

 

click here to enlarge

ibidi µ-Dish Family: Versions and Variations

µ-Dish 35 mm, high       
µ-Dish 35 mm, low µ-Dish 50 mm, low

High walls:
Used for all standard  applications

Low walls:
Provide greater cell access (useful for micromanipulation)

                                         

ibidi Standard Bottom vs. ibidi Glass Bottom


ibidi Standard Bottom

ibidi Glass Bottom

Optical properties
Refractive index (nD 589 nm) 1.52 1.52
Abbe number 56 55
Thickness #1.5 (180 µm) #1.5H (170 µm)
Material Microscopy plastic D 263M Schott borosilicate glass
Autofluorescence Low Low
Transmission Very high (even ultraviolet) High (ultraviolet restrictions)
Birefringence (DIC) Low (DIC compatible*) Low (DIC compatible*)
Other aspects
Surface modifications ibiTreat – tissue culture treated
Uncoated – hydrophobic
Only pure glass
Protein coatings Possible Possible
Gas permeable Yes No
Material flexibility High Low
Breakable No Yes
Recommended for All kinds of fluorescence microscopy TIRF and single photon

* Special glass DIC Lids are available separately


E-mail:
Password:
Forgot your password?
You do not have an account? Register now.

Your shopping cart is empty.

Please note: You need to be logged in to use the shopping cart.