• µ-Slide 8 Well Grid-500
  • µ-Slide 8 Well Grid-500

µ-Slide 8 Well Grid-500

An open µ-Slide (chambered coverslip) with 8 wells and an imprinted 500 µm cell location grid for use in immunofluorescence and high-end microscopy

  • Ideal for locating cells or cell clusters
  • Observation of the sample through the polymer coverslip bottom using high resolution microscopy
  • Cost-effective experiments using small numbers of cells and low volumes of reagents

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Cat. No. Description Pcs./Box
80826-G500 µ-Slide 8 Well Grid-500 ibiTreat: #1.5 polymer coverslip, tissue culture treated, grid repeat distance 500 µm, sterilized 15
80821-G500 µ-Slide 8 Well Grid-500 Uncoated: #1.5 polymer coverslip, hydrophobic, grid repeat distance 500 µm, sterilized 15

Applications:

  • Locating cells or cell clusters, e.g., transfected cells for clone picking
  • Counting events per defined area (e.g., for calculating transfection efficiency)
  • Providing a reference structure for cell movements
  • Cultivation and microscopy of cell cultures
  • Transfection assays 
  • Fluorescence microscopy of living and fixed cells
  • Live cell imaging over extended time periods
  • Suitable for DIC, when used with a DIC lid


Technical Features:

  • Grid with a 500 µm repeat distance
  • Lettered and numbered fields (A-K; 1-10)
  • Open µ-Slide (chambered coverslip) with 8 independent wells
  • Grid clearly visible in phase contrast and bright field microscopy; slightly visible in fluorescence mode
  • Excellent optical quality imaging chamber for high-end microscopy
  • Compatible with staining and fixation
  • ibiTreat surface for optimal cell adhesion
  • Biocompatible plastic material — no glue, no leaking
 

Specifications:

Geometry of the Grid-500

Number of view fields 100
Repeat distance 500 µm
Groove width 5 µm +/- 1 µm
Groove depth < 5 µm

Geometry of the µ-Slide 8 Well

Number of wells 8
Dimensions of wells (w x l x h) in mm 9.4 x 10.7 x 6.8
Volume per well 300 µl
Total height with lid 8 mm
Growth area per well 1.0 cm²
Coating area per well 2.20 cm²
Bottom: ibidi Polymer Coverslip



Layout Grid-500:


Astrid König, Senckenberg Museum of Natural History, Görlitz, Germany

We use the μ-Slide 8 Well Grid-500 for tracking experiments of tardigrades and nematodes. We are really thrilled about the product. The scaling of the slide helps us very much in evaluating the movement of the animals during the experiment.
Please find more details at:
http://onlinelibrary.wiley.com/doi/10.1111/zoj.12435/full

Astrid König
Senckenberg Museum of Natural History
Görlitz, Germany


Sofia Oke, University of Toronto, Department of Immunology, Toronto, Canada

“I have had the chance to try the µ-Slide 8 Well Grid-500 and have been very impressed. I have been using the slides to perform assays with 3D organoid cultures. The µ-Slide 8 Well Grid-500 was very useful and easy for me to monitor my organoids as they grew. I was performing an assay where I irradiated my organoids and monitored recovery. I am very impressed with these products and will be sure to keep in touch if I once again need them in the months to come. 

Sofia Oke
University of Toronto, Department of Immunology
Toronto, Canada

http://www.immunology.utoronto.ca/


John Fassett, University of Graz, Austria

"The µ-Slides 8 Well are great! I really like the ones with the grid. We were able to use them for the live cell imaging of transfected GFP and RFP fusion proteins and could see some really good images.”

John Fassett
University of Graz
Austria

http://www.uni-graz.at/

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