• µ-Slide 4 Well <sup>Ph+</sup>
  • µ-Slide 4 Well <sup>Ph+</sup>

µ-Slide 4 Well Ph+

An open µ-Slide (chambered coverslip) with 4 wells and a special intermediate plate for excellent phase contrast and high-end fluorescence microscopy

  • Excellent phase contrast over the entire well – no meniscus
  • All-in-one chamber slide used for cell culture and microscopy
  • Observation of the sample through the coverslip-like bottom using high resolution microscopy
  • Fast and easy immunofluorescence, with no coverslips and no leakage

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Cat. No. Description Pcs./Box
80446 µ-Slide 4 Well Ph+, ibiTreat: #1.5 polymer coverslip, tissue culture treated, sterilized 15
80442 µ-Slide 4 Well Ph+, Collagen IV: #1.5 polymer coverslip, sterilized 15
80444 µ-Slide 4 Well Ph+, Poly-L-Lysine: #1.5 polymer coverslip, sterilized 15
80441 µ-Slide 4 Well Ph+, Uncoated: #1.5 polymer coverslip, hydrophobic, sterilized 15

Applications:

  • Cultivation and microscopy of cell cultures
  • Transfection assays
  • Fluorescence microscopy of both living and fixed cells
  • Live cell imaging over extended time periods


Technical Features:

  • Open µ-Slide with 4 independent wells
  • Intermediate plate in each well to avoid meniscus formation
  • Easy filling, without air bubbles, through the lateral slot
  • Excellent optical quality for high end microscopy
  • Compatible with staining and fixation
  • ibiTreat surface for optimal cell adhesion
  • Made of biocompatible plastic material – no glue and no leaking
  • Also available with a glass bottom
 

Specifications:

Number of wells 4
Dimensions of wells
(w x l x h) in mm
21.2 x 11.0 x 3.0
Volume per well 700 µl
Total height with lid 10.8 mm
Liquid height 3.0 mm
Growth area per well 2.2 cm²
Coating area per well 5.9 cm²
Bottom: ibidi Polymer Coverslip



Phase contrast microscopy is the most commonly used, transmitted light technique in cell culture. When working with phase contrast microscopy, it is crucial to have the two phase rings adjusted to each other. In open wells, the meniscus at the air-water-interphase works like a lens that refracts the beam path. This miscalibrates the phase rings, leading to poor contrast in the microscopic image.

Working with the µ-Slide 4 Well Ph+ diminishes the meniscus, so that the whole optical system is aligned, no matter which position of the well is being imaged.

µ-Slide 4 Well

µ-Slide 4 Well Ph+

Top View

 

 


The µ-Slide 4 Well Ph+ (Phase Contrast +) is designed for excellent optical quality, especially for cell culture when normal phase contrast microscopy is being used. Opposed to the classic µ-Slide 4 Well, the Ph+ version provides a special intermediate plate in the center of the well. This plate flattens the meniscus that disturbs the phase contrast effect in normal open wells. Openings near the corners provide access to the wells for easy filling and aspiration of liquids.

This innovative technique supports meniscus-free phase contrast microscopy in a very convenient manner. 


Working with the µ-Slide 4 Well Ph+ diminishes the meniscus and increases the area of well contrasted cells

 




The illustration on the left shows the perturbing effect of a meniscus. Light is refracted on the air-water-interface, leading to poor contrast in microscopy. Only the small center part exhibits satisfying phase contrast.

Working with the µ-Slide 4 Well Ph+ diminishes the meniscus and increases the area of nicely contrasted cells. This nice contrast is due to the parallel beam path that is created by the plate.


Comparison Ph+ Well versus Standard Well:
No Meniscus Effect in Ph+ Well

 



 
µ-Slide 4 Well Ph+
Excellent Phase Contrast
Excellent Fluorescence Microscopy

 


Standard Well
Poor Phase Contrast
Excellent Fluorescence Microscopy


Excellent Phase Contrast in the µ-Slide 4 Well Ph+


Click to enlarge

   


Barbara Fogli, Medical University Innsbruck, Austria

"I tested the µ-Slide 4 Well for the culturing of mice DRG neurons. I found them particularly useful for my experiments, since they allow me to have different conditions within the same slide, and to directly do staining and microscopy within the same slide.

After testing them, I decided to order a box of the µ-Slide 4 Well Ph+, because the plastic layer in the middle of the wells allows me to have a more homogeneous distribution of the neurons, which is optimal for microscopy.”

Barbara Fogli
Medical University Innsbruck
Austria

http://www.neuroanatomie.at/

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