• micro-Insert 4 Well FulTrac
  • micro-Insert 4 Well FulTrac

micro-Insert 4 Well FulTrac

A 4-well silicone insert used for tracing individual cells (adherent and suspension)

  • Allows for long-term microscopy assays of single cells
  • Cultivation of small numbers of cells without evaporation risks
  • Conical wells for superb optical quality
  • Complete coverage of the well using a 20x objective

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Cat. No. Description Pcs./Box
80486 micro-Insert 4 Well FulTrac in µ-Dish 35 mm, high ibiTreat: ready to use, tissue culture treated, sterilized 30
80489 25 micro-Inserts 4 Well FulTrac for self-insertion: in a 10 cm transport dish, sterilized 25

Applications:


Technical Features:

  • Four small Ø 0.4 mm microscopy wells
  • Suspension cells cannot escape the observation field
  • Adhesive underside
  • Transferable to any flat, clean surface
  • No material is left behind after the insert is removed
  • Includes a rim for easy handling of the insert with tweezers
 

Cultivation of IVF mouse eggs in the micro-Insert 4 Well FulTrac (prototype; total imaging duration: 12d 17h 55m)
Courtesy of Dr. Asako Sakaue-Sawano and Dr. Atsushi Miyawaki, RIKEN, Japan
(Find more detailed information here.)

Specifications:

Number of wells 4
Dimensions of wells in mm Ø 0.4
Diameter of the complete insert 12 mm
Height of the complete insert 4.3 mm
Volume per well 10 µl
Growth area per well 0.0012 cm2
Coating area per well 0.188 cm2
Material Biocompatible silicone
Bottom No bottom –
sticky underside

Product Selection Guide

 

Cross Section


Ideal Coverage Using Digital Imaging with CCD Cameras

 


Product Variations:

 

Ready-to-use – i.e., transfer of inserts not required

Single micro-Insert 4 Well FulTrac in a 35 mm µ-Dish

  • Individual inserts for single experiments
  • Ideal cell growth on ibiTreat surface
  • Full tracking of individual cells
 

For specialized use – the transfer of inserts is required

25 micro-Insert 4 Well FulTrac for self-insertion

  • 25 pieces in a transport dish
  • Used for self-insertion into 6 or 12 well plates, or other formats
  • Not to be used without transferring the micro-Insert


Possible Filling Procedures:

Minor Well Filling

  • Minimal volume
  • Individual wells

Whole Insert Filling

  • Moderate volume
  • Wells connected by medium

Whole µ-Dish Filling

  • Maximum volume
  • Maximum experimental duration

Evaporation Barrier


The micro-Insert 4 Well FulTrac was developed in cooperation with the RIKEN Brain Science Institute, Japan.
Reference Publication:
Sakaue-Sawano A, Hoshida T, Yo M, Takahashi R, Ohtawa K, Arai T, Takahashi E, Noda S, Miyoshi H and Miyawaki A. Visualizing developmentally programmed endoreplication in mammals using ubiquitin oscillators. The Company of Biologists Ltd | Development (2013) 140, 4624-4632
http://dev.biologists.org/content/develop/140/22/4624.full.pdf


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