• Immunofluorescence Chambers
  • Immunofluorescence Chambers

Immunofluorescence Chambers

Removable silicone cultivation chambers for cell culture and immunofluorescence staining

  • Perfect for immunofluorescence staining and long-term sample storage
  • Cost effective classic immunofluorescence assays
  • Large growth area combined with a small staining volume (especially 3 Well Chamber, removable)
  • Suitable for use with upright and inverted microscopes

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Cat. No. Description Pcs./Box
80381 3 Well Chamber, removable: microscopy glass slide, sterilized 15
80841 8 Well Chamber, removable: microscopy glass slide, sterilized 15
81201 12 Well Chamber, removable: microscopy glass slide, sterilized 15
10811 Coverslips for Chambers, removable: No. 1.5H (170 µm +/- 5 µm) D 263 M Schott glass , 24 mm x 60 mm, unsterile 100
10815 Coverslips and Coverslip Pick-Up Tool for 3 Well Chamber, removable: # 1.5H (170 µm+/- 5 µm) D 263 M Schott glass, Ø 15 mm, unsterile, including one silicone rubber tool for convenient glass coverslip handling 100


  • Immunofluorescence assays using standard coverslip techniques
  • Long-term sample storage
  • 3, 8, or 12 fold sample preparation of adherent cells and tissue samples

Technical Features:

  • Self-adhesive silicone gasket mounted on a standard glass slide
  • Biocompatible silicone material
  • Non-fluorescent glass slide with frosted ends for easy handling and labeling
  • Suitable for use with upright and inverted microscopes, after staining and mounting with a coverslip
  • Staining volume reduction by glass coverslip in 3 Well Chamber, removable
  • Compatible with all common mounting media
  • Optional: The gasket may be transferred to any flat clean surface for cell cultivation


Number of wells 3 8 12
Dimensions of wells (w x l x h) in mm 16.5 x
16.5 x 8.0
12 x
7.75 x 8.0
7.5 x
7.5 x 8
per well
1100 µl
(150 µl*)
400 µl 250 µl
Growth area
per well
1.66 cm² 0.93 cm² 0.56 cm²
Coating area
per well
3.73 cm² 2.63 cm² 1.90 cm²
Material Biocompatible silicone
Suitable coverslip size 24 mm x 60 mm
Bottom      Glass slide (26 x 76 x 1 mm)

* Volume reduction for staining when using a 15 mm glass coverslip (Cat. No. 10815)

Define and print your experimental setup

Standard Procedure 3 Well | 8 Well | 12 Well Chamber, removable:

Low Volume Staining Procedure 3 Well Chamber, removable:

Staining Protocol:

Max-Joseph Kraus, Praxis Geiselgasteig, Grünwald, Germany

"I really love these slides! They are the best that happened to my research workflow. I mainly use the removable 12-well chambers for the high resolution dark field microscopy of platelets and they work excellent for me."

Max-Joseph Kraus
Praxis Geiselgasteig
Grünwald, Germany

Petra Weber, Aalen University, Optical Engineering, Aalen, Germany

"We have tested the Removable Chambers (3 Well and 8 Well) and were very happy. Due to our very sensitive TIRF microscopy with an upright microscope we need appropriate glass slides with removable chambers. We tested adherent HeLa cells to detect Förster Resonance Energy Transfer between EGFR-CFP and Grb2-YFP with Fluorescence Lifetime Imaging. In particular for the activation or inhibition of intracellular signaling pathways, we need the subdivisions on the object slide. It was very easy to remove the chambers. Thank you very much for the possibility to test the chambers for free. I am sure I will use them again."

Petra Weber
Aalen University, Optical Engineering
Aalen, Germany


Daniel Kummer, ZMBE, Institute for Medical Biochemistry, Münster, Germany

"I tested the 8 Well Chamber, removable, and was very happy with the results. The system worked flawlessly. The cells grew very well and the immunofluorescence stainings were of good quality. In general, our institute has always had good experiences with ibidi products. It was the same with the 8 Well Chamber, removable. I have already recommended it to my colleagues."

Daniel Kummer
ZMBE, Institute for Medical Biochemistry
Münster, Germany

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