Culture-Insert Family

Silicone inserts with a defined cell-free gap, suitable for wound healing, migration assays, 2D invasion assays and co-cultivation of cells

Complete solution for wound healing experiments, requiring only a few steps from sample preparation to image analysis
Reproducible experiments owing to: a defined 500 µm cell free gap, no leakage during cultivation, and no material being left behind after the insert’s removal

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Cat. No.	Description	Pcs./Box
80206	µ-Dish 35 mm, low Culture-Insert, ready to use in a µ-Dish 35 mm, low wall, ibiTreat, tissue culture treated, sterile	30
80201	µ-Dish 35 mm, low Culture-Insert, ready to use in a µ-Dish 35 mm, low wall, hydrophobic, uncoated, sterile	30
81176	µ-Dish 35 mm, high Culture-Insert, Culture-Inserts ready to use in a µ-Dish 35 mm, high wall, ibiTreat, tissue culture treated, sterile	30
81171	µ-Dish 35 mm, high Culture-Insert, Culture-Inserts ready to use in a µ-Dish 35 mm, high wall, hydrophobic, uncoated, sterile	30
80241	Culture-Insert 24, a 24 well plate with 24 ready to use Culture-Inserts, tissue culture treated, polystyrene, sterile	3
80209	25 Culture-Inserts for self-insertion, sterile, in a 10 cm transport dish	25

Applications:

Specifications:

Number of wells	2
Outer dimensions (w x l x h) in mm	8.4 x 8.4 x 5
Volume per well	70 µl
Growth area per well	0.22 cm²
Coating area per well	0.82 cm²
Width of cell free gap	500 µm +/- 50 µm
Material	Biocompatible silicone
Bottom	No bottom - sticky underside

Principle for Wound Healing and Migration Assays

Various Applications

A) 2D Invasion Assays

B) Co-Cultivation of Cells

Product Variations: Ready-to-use – i.e., transfer of inserts not required
Single Culture-Insert in a 35 mm µ-Dish Individual inserts for single experiments Ideal cell growth on ibiTreat surface An uncoated version of the µ-Dish is available, allowing for the application of individual coatings and subsequent re-insertion of the Culture-Insert
Culture-Insert 24 For screening applications High parallelization Plate made of polystyrene – i.e., it is not suitable for either fluorescence or high resolution microscopy
*For specialized use – transfer of inserts required*
25 Culture-Inserts for self-insertion 25 pieces in a transport dish Used for self insertion into 6 or 12 well plates, or other formats Not to be used without transferring the Culture-Inserts

Quantitative Wound Healing Image Analysis with WimScratch

This Wound Healing Image Analysis solution evaluates 2D cell migration and provides results in only four steps.

This web-based automated solution generates results within minutes. No extra hardware or software is required. Simply upload your data to the image analysis platform and instantly receive your results via E-mail.

Analysis data contain:

Cell-covered area
Speed of closure (available ≥ 5 images)
Acceleration characteristics (available ≥ 5 images)
Overview chart
Center piece approximation (available ≥ 10 images)

in cooperation with

Culture-Inserts vs. Scratch Assay:
Two assays that are not 100% comparable!


The ibidi Culture-Insert provides improved reproducibility, compared to a Scratch Assay. Data provided by M. Börries, University Freiburg, Germany.
ibidi Culture-Inserts	Scratch Assay
Cell-seeding into designated areas	Scratching the cell surface with a needle or pipet tip
Defined cell-free gap	Varying cell-free gap – i.e., not reproducible
Defined non-coated surface (i.e., ibiTreat or uncoated)	Might contain extra-cellular matrix remains
No cell damage	Cell damage
Internal reference*	No internal reference*

*Internal Reference
The internal reference may answer the question as to whether two opposite cell fronts influence each other or not. With the defined cell seeding technique, it is possible to measure the speed of:
A: a cell front that is opposite another cell front; and
B: a single cell front that does not have an opposite cell front.