• Culture-Insert Family
  • Culture-Insert Family

Culture-Insert Family

Silicone inserts with a defined cell-free gap, suitable for wound healing, migration assays, 2D invasion assays and co-cultivation of cells

  • Complete solution for wound healing experiments, requiring only a few steps from sample preparation to image analysis
  • Reproducible experiments owing to: a defined 500 µm cell free gap, no leakage during cultivation, and no material being left behind after the insert’s removal

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Cat. No. Description Pcs./Box
80206 Culture-Insert in µ-Dish 35 mm, low, ibiTreat: ready to use, tissue culture treated, sterilized 30
81176 Culture-Insert in µ-Dish 35 mm, high, ibiTreat: ready to use, tissue culture treated, sterilized 30
80241 Culture-Insert 24, ibiTreat: a µ-Plate 24 Well with 24 ready to use Culture-Inserts, tissue culture treated, sterilized 3
80209 25 Culture-Inserts for self-insertion: in a 10 cm transport dish, sterilized 25

Applications:

 

Specifications:

Number of wells 2
Outer dimensions
(w x l x h) in mm
8.4 x 8.4 x 5
Volume per well 70 µl
Growth area per well 0.22 cm²
Coating area per well 0.82 cm²
Width of cell free gap 500 µm +/- 50 µm
Material Biocompatible silicone
Bottom No bottom - sticky underside


Principle for Wound Healing and Migration Assays


Various Applications

A) 2D Invasion Assays

B) Co-Cultivation of Cells


Product Variations:

Ready-to-use – i.e., transfer of inserts not required

 

Single Culture-Insert in a 35 mm µ-Dish

  • Individual inserts for single experiments
  • Ideal cell growth on ibiTreat surface
  • An uncoated version of the µ-Dish is available, allowing for the application of individual coatings and subsequent re-insertion of the Culture-Insert

Culture-Insert 24

  • For screening applications
  • High parallelization
  • Based on µ-Plate 24 well – i.e., suitable for fluorescence and high resolution microscopy




For specialized use – transfer of inserts required
 

25 Culture-Inserts for self-insertion

  • 25 pieces in a transport dish
  • Used for self insertion into 6 or 12 well plates, or other formats
Not to be used without transferring the Culture-Inserts


Quantitative Wound Healing Image Analysis with WimScratch 

This Wound Healing Image Analysis solution evaluates 2D cell migration and provides results in only four steps.



This web-based automated solution generates results within minutes. No extra hardware or software is required. Simply upload your data to the image analysis platform and instantly receive your results via E-mail.

Analysis data contain:
  • Cell-covered area
  • Speed of closure (available ≥ 5 images)
  • Acceleration characteristics (available ≥ 5 images)
  • Overview chart
  • Center piece approximation (available ≥ 10 images)
 

in cooperation with


Culture-Inserts vs. Scratch Assay:
Two assays that are not 100% comparable

The ibidi Culture-Insert provides improved reproducibility, compared to a Scratch Assay. Data provided by M. Börries, University Freiburg, Germany.

ibidi Culture-Inserts Scratch Assay
Cell-seeding into designated areas Scratching the cell surface with a needle or pipet tip
Defined cell-free gap Varying cell-free gap – i.e., not reproducible
Defined non-coated surface (i.e., ibiTreat or uncoated) Might contain extra-cellular matrix remains
No cell damage Cell damage
Internal reference* No internal reference*


*Internal Reference

The internal reference may answer the question as to whether two opposite cell fronts influence each other or not. With the defined cell seeding technique, it is possible to measure the speed of:
A: a cell front that is opposite another cell front; and
B: a single cell front that does not have an opposite cell front.

 



Judith Cathcart, Advanced Optical Microscopy Facility, University Health Network, Toronto, Canada

"The ibidi chambers (Culture-Inserts) we used in our wound healing assays helped to keep the cell free area clean and consistent in size without damaging the ECM or leading edge of the cell fronts, making analysis of results more manageable and comparable. They're convenient and easy to use and their small size helps to conserve precious cells and culture materials. We'd certainly use them again.“

Judith Cathcart
Advanced Optical Microscopy Facility
University Health Network
Toronto
Canada

http://www.uhnresearch.ca/facilities/aomf.htm


Jason W. Bjork, PhD, 3M Corporate Research Materials Laboratory, St. Paul, USA

“The ibidi Culture-Inserts for wound healing assays provide a new line of testing for us. We wouldn't do traditional scratch assays at all because of the inherent variation, but with the new inserts we're able to provide many images to support our research. Our results are repeatable and reliable regardless of which researcher conducts the assay.”

Jason W. Bjork
3M Research Materials Laboratory
St. Paul
USA

www.3M.com


Ross Flockhart, PhD, Stanford School of Medicine, Stanford, USA

"The ibidi cell migration Culture-Inserts enabled me to perform highly reproducible and quantitative migration experiments. Since physical scratching isn't required, uniformity is maintained and adverse wounding responses are not an issue.

The online quantification tool WimScratch for the assay is also fantastic and it yields quantitative data from the images extremely quickly with little effort required. I'd definitely recommend it for migration studies.“

Ross Flockhart, PhD
Stanford School of Medicine
Department of Dermatology
Stanford
USA

http://khavarilab.stanford.edu/

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