• Culture-Insert 4 Well
  • Culture-Insert 4 Well

Culture-Insert 4 Well

4 well silicone insert with 4 defined cell-free gaps and a center migration area, suitable for wound healing, migration assays, 2D invasion assays, and co-cultivation of cells

  • Complete solution for wound healing experiments, requiring only a few steps from sample preparation to image analysis
  • Reproducible experiments owing to: four defined 500 µm cell free gaps and one 1000 µm center gap, no leakage during cultivation, and no material being left behind after the insert’s removal
  • Suitable for 4 experiments, including a center gap that allows to observe the migration of all cell types

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Cat. No. Description Pcs./Box
80466 Culture-Insert 4 Well in µ-Dish 35 mm, high ibiTreat: ready to use, tissue culture treated, sterilized 30
80469 25 Culture-Inserts 4 Well for self-insertion: in a 10 cm transport dish, sterilized 25

Applications:

 

Specifications:

Number of wells 4
Outer dimensions Ø 17 mm
Volume per well 110 µl
Growth area per well 0.35 cm²
Coating area per well 1.23 cm²
Width of cell free gap  
- Two cell fronts 500 µm +/- 50 µm
- Four cell fronts (center) 1000 µm +/- 100 µm
Material Biocompatible silicone
Bottom No bottom - sticky underside


Principle for Wound Healing and Migration Assays


Step 1: cell seeding and attachment

Step 2: remove insert after cultivation

Step 3:
overlay patches with culture medium



Various Applications



Wound Healing / Migration

The Culture-Insert 4 Well allows for wound healing and migration assays with four cell-free gaps with a 0.5 mm gap width, and one center area with a 1 mm diagonal distance. Compared to the 2 well version, more cells can be analyzed and a higher throughput is realized. Two cell-free gaps create a technical replicate in the very same well.



Two Conditions of Wound Healing / Migration

With the Culture-Insert 4 Well, a wound healing experiment and a control experiment can be done in the same well. The control cells can be pre-treated with any kind of treatment (e.g., drugs, plasmids, mRNA, or virus particles).



Four Conditions for Co-Cultivation / Invasion

By seeding up to four different cell types, the invasiveness of adherent cells can be analyzed. Non-invasive cell types can be cultured next to each other, sharing the same medium. This co-culture allows the cells to communicate and share factors for differentiation studies or long-term culturing.

Invasion and co-cultivated cells can be imaged by unsing high-resolution fluorescence microscopy.


Visualization of Cell Migration

Click here to watch our movie


Product Variations:

Ready-to-use – i.e., transfer of inserts not required

 

Single Culture-Insert 4 Well in a 35 mm µ-Dish

  • Individual inserts for single experiments
  • Ideal cell growth on ibiTreat surface
  • An uncoated version of the µ-Dish is available, allowing for the application of individual coatings and subsequent re-insertion of the Culture-Insert

For specialized use – transfer of inserts required  

25 Culture-Inserts 3 Well for self-insertion

  • 25 pieces in a transport dish
  • Used for self-insertion into 6 or 12 well plates, or other formats
Not to be used without transferring the Culture-Inserts


Only 4 Steps from Sample Preparation to Image Analysis







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