• Culture-Insert 3 Well
  • Culture-Insert 3 Well

Culture-Insert 3 Well

3 well silicone insert with two defined cell-free gaps, suitable for wound healing, migration assays, 2D invasion assays, and co-cultivation of cells

  • Complete solution for wound healing experiments, requiring only a few steps from sample preparation to image analysis
  • Reproducible experiments owing to: two defined 500 µm cell free gaps, no leakage during cultivation, and no material being left behind after the insert’s removal
  • Suitable for 3 experiments in parallel

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Cat. No. Description Pcs./Box
80366 Culture-Insert 3 Well in µ-Dish 35 mm, high ibiTreat: ready to use, tissue culture treated, sterilized 30
80369 25 Culture-Inserts 3 Well for self-insertion: in a 10 cm transport dish, sterilized 25

Applications:

 

Specifications:

Number of wells 3
Outer dimensions
(w x l x h) in mm
8.4 x 12.15 x 5
Volume per well 70 µl
Growth area per well 0.22 cm²
Coating area per well 0.82 cm²
Width of cell free gap 500 µm +/- 50 µm
Material Biocompatible silicone
Bottom No bottom - sticky underside


Principle for Wound Healing and Migration Assays


Step 1: cell seeding and attachment

Step 2: remove insert after cultivation

Step 3:
overlay patches with culture medium



Various Applications



Wound Healing / Migration

Wound healing and migration assays are done by seeding cells into the Culture-Insert 3 Well. After cell attachment, two cell-free gaps are created in which the cell migration can be visualized. Compared to the 2 well version, more cells can be analyzed and a higher throughput is achieved. Two cell-free gaps create a technical replicate in the very same plate or dish.



Two Conditions of Wound Healing / Migration

With the Culture-Insert 3 Well, a wound healing experiment and a control experiment can be done in the same well. The control cells can be pre-treated with any kind of treatment (e.g., drugs, plasmids, mRNA, or virus particles).



Co-Cultivation / Invasion

By seeding two different cell types, the invasiveness of adherent cells can be analyzed. Non-invasive cell types can be cultured next to each other, sharing the same medium. This co-culture allows the cells to communicate and share factors for differentiation studies or long-term culturing.

Invasion and co-cultivated cells can be imaged by high-resolution fluorescence microscopy.


Visualization of Cell Migration

Click here to watch our movie


Product Variations:

Ready-to-use – i.e., transfer of inserts not required

 

Single Culture-Insert 3 Well in a 35 mm µ-Dish

  • Individual inserts for single experiments
  • Ideal cell growth on ibiTreat surface
  • An uncoated version of the µ-Dish is available, allowing for the application of individual coatings and subsequent re-insertion of the Culture-Insert

For specialized use – transfer of inserts required  

25 Culture-Inserts 3 Well for self-insertion

  • 25 pieces in a transport dish
  • Used for self-insertion into 6 or 12 well plates, or other formats
Not to be used without transferring the Culture-Inserts


Only 4 Steps from Sample Preparation to Image Analysis







Henrike Rebl, University Medicine, Rostock, Germany

"I have tested the Culture-Insert 2 Well and 3 Well and they worked quite nicely. I will definitely use the ibidi Culture-Inserts and µ-Dishes again because they are so easy to use and they work great for fluorescence microscopy."

Henrike Rebl
University Medicine
Rostock, Germany
https://www.med.uni-rostock.de

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