• µ-Slide 4 Well <sup>Ph+</sup> Glass Bottom
  • µ-Slide 4 Well <sup>Ph+</sup> Glass Bottom

µ-Slide 4 Well Ph+ Glass Bottom

Open µ-Slide with 4 wells with a glass bottom and a special intermediate plate for excellent phase contrast, suitable for use in TIRF and single molecule applications

  • Perfect cell imaging thanks to the low thickness variability of the coverslips’ glass
  • Cost-effective experiments using small numbers of cells and low volumes of reagents
  • Excellent phase contrast over the entire well – no meniscus

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Cat. No. Description Pcs./Box
80447 µ-Slide 4 Well Ph+ Glass Bottom: No. 1.5H (170 µm +/- 5 µm) D 263 M Schott glass, sterilized 15

Applications

  • Cultivation and microscopy of cell cultures
  • TIRF and single molecule applications


Technical Features

  • Open µ-Slide with 4 independent wells
  • Intermediate plate in each well to avoid meniscus formation
  • Bottom made from D 263 M Schott glass, No. 1.5H
    (170 +/- 5 µm)
  • May require coating to promote cell attachment
  • Also available as a µ-Slide 4 Well Ph+ with an ibidi Standard Bottom for superior cell growth
 

Specifications:

Number of wells 4
Dimensions of wells
(w x l x h) in mm
21.2 x 11.0 x 3.0
Volume per well 700 µl
Liquid height 3.0 mm
Total height with lid 10.8 mm
Growth area per well 2.2 cm²
Coating area per well 5.9 cm²
Bottom: Glass coverslip No. 1.5H, selected quality, 170 µm +/- 5 µm


ibidi Polymer Coverslip versus ibidi Glass Bottom:


ibidi Polymer Coverslip

ibidi Glass Bottom

Optical properties

Refractive index (nD 589 nm) 1.52 1.52
Abbe number 56 55
Thickness #1.5 (180 µm) #1.5H (170 µm)
Material Microscopy plastic D 263M Schott borosilicate glass
Autofluorescence Low Low
Transmission Very high (even ultraviolet) High (ultraviolet restrictions)
Birefringence (DIC) Low (DIC compatible*) Low (DIC compatible*)

Other aspects

Surface modifications ibiTreat – tissue culture treated
Uncoated – hydrophobic
Only glass
Protein coatings Compatible Compatible
Gas permeable Yes No
Material flexibility High Low
Breakable No Yes
Applications Fluorescence microscopy TIRF and single photon

* Special glass DIC lids for µ-Dishes and open µ-Slides are available separately.


Phase contrast microscopy is the most commonly used, transmitted light technique in cell culture. When working with phase contrast microscopy, it is crucial to have the two phase rings adjusted to each other. In open wells, the meniscus at the air-water-interphase works like a lens that refracts the beam path. This miscalibrates the phase rings, leading to poor contrast in the microscopic image.

Working with the µ-Slide 4 Well Ph+ diminishes the meniscus, so that the whole optical system is aligned, no matter which position of the well is being imaged.

µ-Slide 4 Well
Glass Bottom

µ-Slide 4 Well Ph+
Glass Bottom

Top View

 

 


The µ-Slide 4 Well Ph+ Glass Bottom (Phase Contrast +) is designed for excellent optical quality, especially for cell culture when normal phase contrast microscopy is being used. Opposed to the classic µ-Slide 4 Well Glass Bottom, the Ph+ version provides a special intermediate plate in the center of the well. This plate flattens the meniscus that disturbs the phase contrast effect in normal open wells. Openings near the corners provide access to the wells for easy filling and aspiration of liquids.

This innovative technique supports meniscus-free phase contrast microscopy in a very convenient manner. 


Working with the µ-Slide 4 Well Ph+ Glass Bottom diminishes the meniscus and increases the area of well contrasted cells

 




The illustration on the left shows the perturbing effect of a meniscus. Light is refracted on the air-water-interface, leading to poor contrast in microscopy. Only the small center part exhibits satisfying phase contrast.

Working with the µ-Slide 4 Well Ph+ Glass Bottom diminishes the meniscus and increases the area of nicely contrasted cells. This nice contrast is due to the parallel beam path that is created by the plate.


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