• µ-Slide 2 Well Glass Bottom
  • µ-Slide 2 Well Glass Bottom

µ-Slide 2 Well Glass Bottom

Open µ-Slide with 2 wells with a glass bottom, suitable for use in TIRF and single molecule applications

  • Perfect cell imaging thanks to the low thickness variability of the coverslips’ glass
  • Cost-effective experiments using small numbers of cells and low volumes of reagents

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Cat. No. Description Pcs./Box
80287 µ-Slide 2 Well Glass Bottom: No. 1.5H (170 µm +/- 5 µm) D 263 M Schott glass, sterilized 15

Applications

  • Cultivation and microscopy of cell cultures
  • TIRF and single molecule applications


Technical Features

  • Open µ-Slide with 2 independent wells
  • Bottom made from D 263 M Schott glass, No. 1.5H
    (170 +/- 5 µm)
  • May require coating to promote cell attachment
  • Also available in a Ph+ version: intermediate plate in each well to avoid meniscus formation
  • Also available as a µ-Slide 2 Well with an ibidi Standard Bottom for superior cell growth
 

Specifications:

Number of wells 2
Dimensions of wells
(w x l x h) in mm
21.2 x 23.3 x 9.3
Volume per well 1500 µl
Total height with lid 10.8 mm
Growth area per well 4.8 cm2
Coating area per well 7.5 cm²
Bottom: Glass coverslip No. 1.5H, selected quality,
170 µm +/- 5 µm


ibidi Polymer Coverslip versus ibidi Glass Bottom:


ibidi Polymer Coverslip

ibidi Glass Bottom

Optical properties

Refractive index (nD 589 nm) 1.52 1.52
Abbe number 56 55
Thickness #1.5 (180 µm) #1.5H (170 µm)
Material Microscopy plastic D 263M Schott borosilicate glass
Autofluorescence Low Low
Transmission Very high (even ultraviolet) High (ultraviolet restrictions)
Birefringence (DIC) Low (DIC compatible*) Low (DIC compatible*)

Other aspects

Surface modifications ibiTreat – tissue culture treated
Uncoated – hydrophobic
Only glass
Protein coatings Compatible Compatible
Gas permeable Yes No
Material flexibility High Low
Breakable No Yes
Applications Fluorescence microscopy TIRF and single photon

* Special glass DIC lids for µ-Dishes and open µ-Slides are available separately.


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