• µ-Dish<sup> 35 mm, high</sup> glass bottom Grid-500
  • µ-Dish<sup> 35 mm, high</sup> glass bottom Grid-500

µ-Dish 35 mm, high glass bottom Grid-500

A 35 mm imaging dish with a glass bottom and an imprinted 500 µm relocation grid

  • Ideal for relocating cells or cell clusters
  • Easy cell counting per defined area
  • Glass coverslip for TIRF and single molecule applications

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Cat. No. Description Pcs./Box
81168 µ-Dish 35 mm, high glass bottom, Grid-500, Ø 35 mm, high wall, 2 ml volume, No. 1.5H (170 µm +/- 5 µm) D 263 M Schott glass, sterile, grid repeat distance 500 µm 30


  • Relocating cells or cell clusters, e.g., transfected cells for clone picking
  • Counting events per defined area (e.g., when calculating transfection efficiency)
  • Providing a reference structure for cell movements
  • Treatment of distinct single cells, such as in microinjection
  • Following axon growth on a defined scale bar

Technical Features

  • Grid with 500 µm repeat distance
  • Lettered and numbered 4 x 400 squares (A-U; 1-20)
  • Grid and cells in one focal plane
  • Bottom made from D 263 M Schott glass with a thickness of 170 µm +/- 10 µm
  • Lid with lock position, which minimizes evaporation
  • May require coating to promote cell attachment


Grid repeat distance 500 µm
Ø µ-Dish 35 mm
Volume 2 ml
Growth area 3.5 cm2
Coating area using 400 µl 4.1 cm2
Ø observation area 21 mm
Height with / without lid 14/12 mm
Bottom Glass coverslip No. 1.5, selected quality,
170 µm +/- 10 µm

Layout Grid-500

ibidi Standard Bottom vs. ibidi Glass Bottom

ibidi Standard Bottom

ibidi Glass Bottom

Optical properties
Refractive index (nD 589 nm) 1.52 1.52
Abbe number 56 55
Thickness #1.5 (180 µm) #1.5 (170 µm)
Material Microscopy plastic D 263M Schott borosilicate glass
Autofluorescence Low Low
Transmission Very high (even ultraviolet) High (ultraviolet restrictions)
Birefringence (DIC) Low (DIC compatible*) Low (DIC compatible*)
Other aspects
Surface modifications ibiTreat – tissue culture treated
Uncoated – hydrophobic
Only pure glass
Protein coatings Possible Possible
Gas permeable Yes No
Material flexibility High Low
Breakable No Yes
Recommended for All kinds of fluorescence microscopy TIRF and single photon

* Special glass DIC Lids are available separately

James J. Faust and David G. Capco, Arizona State University, Tempe, USA

“ibidi has made the tedious process of correlated light- and scanning electron microscopy  remarkably simplified with their µ-Dishes 35mm, high glass bottom Grid 500. This product combines all of the essential elements for high quality imaging: optically flawless glass essential for high resolution light microscopy, media reservoirs suited for a variety of imaging systems, and a grid system to rapidly relocate specimens in the scanning electron microscope. This product has made the difficult easy.”

James J. Faust and David G. Capco
Molecular and Cellular Biosciences
State University
, AZ


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