µ-Dish^{35 mm, high} glass bottom Grid-500

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Cat. No.	Description	Pcs./Box
81168	µ-Dish 35 mm, high glass bottom, Grid-500, Ø 35 mm, high wall, 2 ml volume, No. 1.5 (170 µm +/- 10 µm) D 263 M Schott glass, sterile, grid repeat distance 500 µm	30

Relocating cells or cell clusters, e.g., transfected cells for clone picking
Counting events per defined area (e.g., when calculating transfection efficiency)
Providing a reference structure for cell movements
Treatment of distinct single cells, such as in microinjection
Following axon growth on a defined scale bar

Grid repeat distance

500 µm

Ø µ-Dish

35 mm

Volume

2 ml

Growth area

3.5 cm²

Coating area using 400 µl

4.1 cm²

Ø observation area

21 mm

Height with / without lid

14/12 mm

Bottom

Glass coverslip No. 1.5, selected quality,
170 µm +/- 10 µm

	ibidi Standard Bottom	ibidi Glass Bottom
Optical properties
Refractive index (n_D 589 nm)	1.52	1.52
Abbe number	56	55
Thickness	#1.5 (180 µm)	#1.5 (170 µm)
Material	Microscopy plastic	D 263M Schott borosilicate glass
Autofluorescence	Low	Low
Transmission	Very high (even ultraviolet)	High (ultraviolet restrictions)
Birefringence (DIC)	Low (DIC compatible*)	Low (DIC compatible*)
Other aspects
Surface modifications	ibiTreat – tissue culture treated Uncoated – hydrophobic	Only pure glass
Protein coatings	Possible	Possible
Gas permeable	Yes	No
Material flexibility	High	Low
Breakable	No	Yes
Recommended for	All kinds of fluorescence microscopy	TIRF and single photon