• µ-Dish<sup> 35 mm, high</sup> glass bottom Grid-50
  • µ-Dish<sup> 35 mm, high</sup> glass bottom Grid-50

µ-Dish 35 mm, high glass bottom Grid-50

A 35 mm imaging dish with a glass bottom and an imprinted 50 µm relocation grid

  • Ideal for relocating cells or cell clusters
  • Easy cell counting per defined area
  • Glass coverslip for TIRF and single molecule applications

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Cat. No. Description Pcs./Box
81148 µ-Dish 35 mm, high, Glass Bottom, Grid-50: Ø 35 mm, high wall (2 ml volume), #1.5H (170 µm +/- 5 µm) D 263 M Schott glass, grid repeat distance 50 µm, sterilized 30

Applications

  • Relocating cells or cell clusters, e.g., transfected cells for clone picking
  • Counting events per defined area (e.g., when calculating transfection efficiency)
  • Providing a reference structure for cell movements
  • Treatment of distinct single cells, such as in microinjection


Technical Features

  • Grid with 50 µm µm repeat distance
  • Lettered and numbered 4 x 400 squares (A-U; 1-20)
  • Grid and cells in one focal plane
  • Bottom made from D 263 M Schott glass with a thickness of 170 µm +/- 5 µm
  • Lid with lock position, which minimizes evaporation
  • May require coating to promote cell attachment
 

Specifications:

Grid repeat distance 50 µm
Ø µ-Dish 35 mm
Volume 2 ml
Growth area 3.5 cm2
Coating area using 400 µl 4.1 cm2
Ø observation area 21 mm
Height with / without lid 14/12 mm
Bottom Glass coverslip No. 1.5H, selected quality,
170 µm +/- 5 µm

Layout Grid-50


ibidi Standard Bottom vs. ibidi Glass Bottom


ibidi Standard Bottom

ibidi Glass Bottom

Optical properties
Refractive index (nD 589 nm) 1.52 1.52
Abbe number 56 55
Thickness #1.5 (180 µm) #1.5H (170 µm)
Material Microscopy plastic D 263M Schott borosilicate glass
Autofluorescence Low Low
Transmission Very high (even ultraviolet) High (ultraviolet restrictions)
Birefringence (DIC) Low (DIC compatible*) Low (DIC compatible*)
Other aspects
Surface modifications ibiTreat – tissue culture treated
Uncoated – hydrophobic
Only pure glass
Protein coatings Possible Possible
Gas permeable Yes No
Material flexibility High Low
Breakable No Yes
Recommended for All kinds of fluorescence microscopy TIRF and single photon

* Special glass DIC Lids are available separately


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