• µ-Dish<sup> 35 mm, high</sup> glass bottom
  • µ-Dish<sup> 35 mm, high</sup> glass bottom

µ-Dish 35 mm, high glass bottom

A 35 mm imaging dish with a glass bottom, suitable for use in TIRF and single molecule applications

  • Perfect cell imaging thanks to the low thickness variability of the coverslips’ glass
  • Lid with lock position, which minimizes evaporation
  • Suitable for DIC, when used with the special DIC Lid
  • Also available with a relocation grid: Grid-50 / Grid-500

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Cat. No. Description Pcs./Box
81158 µ-Dish 35 mm, high glass bottom, Ø 35 mm, 2 ml volume, No. 1.5H (170 µm +/- 5 µm) D 263 M Schott glass, sterile 60

Applications

  • Cultivation and microscopy of cell cultures
  • Fluorescence Correlation Spectroscopy (FCS)
  • TIRF and single molecule applications



 

Specifications:

Ø µ-Dish 35 mm
Volume 2 ml
Growth area 3.5 cm2
Coating area using 400 µl 4.1 cm2
Ø observation area 21 mm
Height with / without lid 14/12 mm
Bottom Glass coverslip No. 1.5H,
selected quality,
170 µm +/- 5 µm

Technical Features

  • Bottom made from D 263 M Schott glass with a thickness of 170 µm +/- 5 µm
  • Standard format dish with a 35 mm diameter and high walls
  • May require coating to promote cell attachment
 

ibidi Standard Bottom vs. ibidi Glass Bottom


ibidi Standard Bottom

ibidi Glass Bottom

Optical properties
Refractive index (nD 589 nm) 1.52 1.52
Abbe number 56 55
Thickness #1.5 (180 µm) #1.5H (170 µm)
Material Microscopy plastic D 263M Schott borosilicate glass
Autofluorescence Low Low
Transmission Very high (even ultraviolet) High (ultraviolet restrictions)
Birefringence (DIC) Low (DIC compatible*) Low (DIC compatible*)
Other aspects
Surface modifications ibiTreat – tissue culture treated
Uncoated – hydrophobic
Only pure glass
Protein coatings Possible Possible
Gas permeable Yes No
Material flexibility High Low
Breakable No Yes
Recommended for All kinds of fluorescence microscopy TIRF and single photon

* Special glass DIC Lids are available separately


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