• µ-Dish<sup> 35 mm, high</sup> Glass Bottom
  • µ-Dish<sup> 35 mm, high</sup> Glass Bottom

µ-Dish 35 mm, high Glass Bottom

A 35 mm imaging dish with a glass bottom, suitable for use in TIRF and single molecule applications

  • Perfect cell imaging thanks to the low thickness variability of the coverslips’ glass (# 1.5H)
  • Lid with lock position, which minimizes evaporation
  • Suitable for DIC, when used with the special DIC Lid
  • Also available with a relocation grid: Grid-50 / Grid-500

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Cat. No. Description Pcs./Box
81158 µ-Dish 35 mm, high, Glass Bottom: Ø 35 mm, high wall (2 ml volume), # 1.5H (170 µm +/- 5 µm) D 263 M Schott glass, sterilized 60

Applications

  • Cultivation and microscopy of cell cultures
  • Fluorescence Correlation Spectroscopy (FCS)
  • TIRF and single molecule applications



 

Specifications:

Ø µ-Dish 35 mm
Volume 2 ml
Growth area 3.5 cm2
Coating area using 400 µl 4.1 cm2
Ø observation area 21 mm
Height with / without lid 14/12 mm
Bottom Glass coverslip No. 1.5H,
selected quality,
170 µm +/- 5 µm

Technical Features

  • Bottom made from D 263 M Schott glass with a thickness of 170 µm +/- 5 µm
  • Standard format dish with a 35 mm diameter and high walls
  • May require coating to promote cell attachment
 

Detailed Comparison ibidi Dishes 35 mm

  µ-Dish 35 mm, high
(Polymer Coverslip, ibiTreat / Uncoated)
81156, 81151
µ-Dish 35 mm, high
Glass Bottom


81158
Glass Bottom Dish 35 mm

81218-200
81218-800
Bottom Thickness 180 µm (+10/-5 µm) 170 µm (+/-5 µm) 170 µm (+20/-10 µm)
Bottom Material #1.5 Polymer coverslip
(ibidi)
#1.5H glass coverslip
(D 263 M Schott high precision glass)
#1.5 glass coverslip
(D 263 M Schott high precision glass)
Lid Lid lock for minimal evaporation and safe handling Lid lock for minimal evaporation and safe handling Standard
Surfaces ibiTreat (tissue culture treated),
Uncoated (hydrophobic)
Uncoated glass Uncoated glass
Gas permeable Yes No No
Packaging Sterile, single-packed Sterile, single-packed Sterile, packed at 10 pcs.
Quantity 60 pcs./box 60 pcs./box 200/800 pcs./box
Free Sample Yes Yes Yes

Microscopy Applications:

High resolution microscopy1
Confocal + + +
Brightfield, Phase Contrast, DIC + + +
Widefield Fluorescence + + +
TIRF - + -
Super-Resolution microscopy2
STED, STORM, etc.  - + -

Optical Properties:

Refractive Index
(nD 589 nm)
1.52 1.52 1.52
Abbe number 55 56 56
Autofluorescence Low Low Low
Transmission Very high (even ultraviolet) High (ultraviolet restrictions) High (ultraviolet restrictions)
Birefringence (DIC) Low (DIC compatible)3 Low (DIC compatible)3 Low (DIC compatible)3

Note:

1High resolution microscopy = high magnification (40x - 100x) and high resolution microscopy including widefield or confocal techniques (e.g., brightfield, DIC, fluorescence). The resolution is limited to ~200 nm by the diffraction limit. Does not include Super-Resolution.
Resolution is described as a function to discriminate 2 dots from each other; it is dependent on wavelength and numerical aperture. It is physically limited by Abbe’s law which can only be overcome by Super-Resolution microscopy.

2Super-Resolution microscopy = additional resolution by overcoming the diffraction limit with 63x - 100x oil immersion objectives. Super-Resolution provides a resolution below 200 nm, down to 5-20nm by computational (PALM, SIM, STORM, RESOLVED) and one optical Super-Resolution technology (STED). TIRF is not a Super-Resolution technology.

³DIC Compatibility = The lids are not DIC compatible. Special glass DIC Lids for the µ-Dishes are available separately.


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