µ-Dish^{35 mm, high} ESS

A 35 mm Petri dish with an Elastically Supported Surface (ESS) used for growing cells under “in vivo-near” conditions

“in vivo-near” cell culture on elastically supported surface
High resolution microscopy on soft cell culture surface
Based on the standard µ-Dish^{35 mm, high}

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Cat. No.	Description	Pcs./Box
81291	µ-Dish 35 mm, high ESS, uncoated elastic surface with a stiffness of 1.5 kPa, sterile, Ø 35 mm, 2 ml volume	30
81391	µ-Dish 35 mm, high ESS, uncoated elastic surface with a stiffness of 15 kPa, sterile, Ø 35 mm, 2 ml volume	30
81191	µ-Dish 35 mm, high ESS, uncoated elastic surface with a stiffness of 28 kPa, sterile, Ø 35 mm, 2 ml volume	30

Instructions

Instructions µ-Dish^{35 mm, high} ESS (PDF)

FAQ

Do cells directly grow on the ESS surface?

Applications

Cell culture under “in vivo-near” conditions, e.g. cardiomyocytes
Cellular mechanics research
Immunofluorescence stainings
Live cell imaging of cell differentiation on soft surface
Transfection
Suitable for DIC , when used with the special DIC Lid

Technical Features

40 μm thick PDMS (Polydimethylsiloxane) layer on top of a 100 μm thick glass cover
Stiffness ranging from 1.5 kPa to 28 kPa, as in most tissues
Biocompatible soft cell culture material
High walls with a standard height for easy handling
Lid with lock position, which minimizes evaporation
Rim for easy opening
No autofluorescence
Fully compatible with the ibidi Heating System

Specifications:

Ø µ-Dish	35 mm
Volume	2 ml
Growth area	3.5 cm²
Coating area using 400 µl	4.1 cm²
Ø observation area	21 mm
Height with / without lid	14/12 mm
Bottom	Elastic surface (40 µm) on glass coverslip (100 µm)

Differentiated mouse fibroblasts cultured on elastic surface (ESS 28 kPa)
green: zyxin (Alexa 488 conjugated antibody)
red: alpha- smooth muscle actin (Cy5 conjugated antibody)
(courtesy R. Merkel, Jülich, Germany)

For a detailed explanation on the ESS technology please refer to the Special Surfaces section of our website.