• µ-Dish<sup> 35 mm, high</sup> ESS
  • µ-Dish<sup> 35 mm, high</sup> ESS

µ-Dish 35 mm, high ESS

A 35 mm imaging dish with an Elastically Supported Surface (ESS) used for growing cells under “in vivo-near” conditions

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Cat. No. Description Pcs./Box
81291 µ-Dish 35 mm, high, ESS 1.5 kPa, Uncoated: Ø 35 mm, high wall (2 ml volume), elastic surface with a stiffness of 1.5 kPa, hydrophobic, sterilized 30
81391 µ-Dish 35 mm, high, ESS 15 kPa, Uncoated: Ø 35 mm, high wall (2 ml volume), elastic surface with a stiffness of 15 kPa, hydrophobic, sterilized 30
81191 µ-Dish 35 mm, high, ESS 28 kPa, Uncoated: Ø 35 mm, high wall (2 ml volume), elastic surface with a stiffness of 28 kPa, hydrophobic, sterilized 30
81199 µ-Dish 35 mm, high, ESS Variety Pack, Uncoated: Ø 35 mm, high wall (2 ml volume), elastic surface with a stiffness of 1.5, 15, and 28 kPa, hydrophobic, sterilized 30

Applications


Technical Features

  • 40 μm thick PDMS (Polydimethylsiloxane) layer on top of a 100 μm thick glass cover
  • Available stiffness: 1.5 kPa, 15 kPa, and 28 kPa
  • Biocompatible soft cell culture material
  • High walls with a standard height for easy handling
  • Lid with lock position, which minimizes evaporation
  • Rim for easy opening
  • No autofluorescence
  • Fully compatible with the ibidi Heating System


 

Specifications:

Ø µ-Dish 35 mm
Volume 2 ml
Growth area 3.5 cm2
Coating area using 400 µl 4.1 cm2
Ø observation area 21 mm
Height with / without lid 14/12 mm
Bottom Elastic surface (40 µm) on
glass coverslip (100 µm)



Differentiated mouse fibroblasts cultured on elastic surface (ESS 28 kPa)
green: zyxin (Alexa 488 conjugated antibody)
red: alpha- smooth muscle actin (Cy5 conjugated antibody)

(courtesy R. Merkel, Jülich, Germany)



For a detailed explanation on the ESS technology please refer to the Special Surfaces section of our website. 


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