• µ-Slide III<sup> 3in1</sup>
  • µ-Slide III<sup> 3in1</sup>

µ-Slide III 3in1

A μ-Slide for switchable chemotatic gradients that merges 3 separated liquids into one channel

  • Dynamic fluidic gradients with a maximum time resolution
  • Alternating gradient fields for chemotactic cell trapping

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Cat. No. Description Pcs./Box
80316 µ-Slide III 3in1, ibiTreat: #1.5 polymer coverslip, tissue culture treated, sterilized 15
80311 µ-Slide III 3in1, Uncoated: #1.5 polymer coverslip, hydrophobic, sterilized 15

Applications:

  • Fluidic assays with up to three different liquids
  • Cell sorting with laser traps
  • Fluidic focusing of inner lane


Technical Features:

  • 3 channels ending in 1
  • Microfluidic bi-directional gradient generator
  • Fully compatible with the ibidi Pump System and other pumps
  • The flow is always laminar
  • Contains a millimeter scale bar
 

Specifications:

Adapters Female Luer
Volume per reservoir 60 µl
Number of channels 3 in 1
Total channel volume 60 µl
Height of all channels 0.4 mm
Width of channels thin/thick 1/3 mm
Total growth area 1.23 cm²
Coating area 3.05 cm²
Distance of scale bars 1 mm
Bottom: ibidi Polymer Coverslip  

Flow Setup for Switchable Chemotactic Gradients

 

 

Gradient steepness at different positions. The flow is generated by the ibidi Pump System.   Overlapping gradients for chemotactical cell trapping. Switching times of some seconds allow fast gradient switching, so that cytoskeletal dynamics can be observed using fluorescence microscopy.

Example Experiment

   


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