• µ-Slide Chemotaxis
  • µ-Slide Chemotaxis

µ-Slide Chemotaxis

Used to investigate chemotaxis of fast or slow migrating adherent cells and non-adherent cells in gel matrices

  • Chemotaxis measurement in real-time
  • Stable gradients for long-term experiments
  • Reproducible results with reliable and user-independent data

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Cat. No. Description Pcs./Box
80326 µ-Slide Chemotaxis, ibiTreat: #1.5 polymer coverslip, tissue culture treated, sterilized 10
80322 µ-Slide Chemotaxis, Collagen IV: #1.5 polymer coverslip, sterilized 10

Applications:

  • Chemotaxis of neutrophiles, lymphocytes, and monocytes
  • 3D chemotaxis of leukoycytes or cancer cells in ECM-like matrix
  • Chemotaxis experiments of fast or slow migrating cells
  • Invasion assays of tumor cells in Matrigel™


Technical Features:

  • Chamber geometry optimized for cells in 3D matrices
  • 3 chambers on one slide for parallel assays
  • Ideal for collagen gels, hydrogels, Matrigel™, or similar aqueous gels
  • Ready to use, i.e., no assembly required
  • Lettered and numbered chambers and reservoirs
 

Specifications:

Chemotaxis chambers on slide 3
Volume per chamber 120 µl
Observation area 2x1 mm²
Coating area per chamber
- When coating full chamber
- When coating observation area only

3.50 cm2
0.27 cm2
Distance between chambers 18.5 mm
Total height with plugs 12 mm
Volume chemoattractant 30 µl
Bottom: ibidi Polymer Coverslip  


Define and print your experimental setup

Application Examples

Cells, embedded into a gel, migrating towards a chemoattractant.

 

Dendritic cell (mouse) with LifeAct-labeled F-actin that is migrating towards CCL 19 inside a Collagen I gel matrix. Spinning disc confocal microscopy (objective lens 63 x).

 

HT-1080 cells migrating towards FCS inside a gel matrix (objective lens 10 x).

Necessary Equipment

Basic requirements:

  • Inverted microscope with a 5x objective and 10x phase contrast
  • Basic camera for time lapse movies
  • Heated microscopy system (required for experimentation on most mammalian cell types) 
 

 

Recommended extensions:


Data Analysis

Chemotaxis and Migration Tool

To facilitate analysis of results from chemotaxis experiments (time stacks) ibidi has developed this software analysis tool. The tool provides various types of graphs and statistical tests for advanced analysis of experimental data.

More >>


Loïc Dupré, INSERM, Toulouse, France

"The ibidi µ-Slides Chemotaxis have been the key to my research on lymphocyte chemotaxis. These slides have allowed me to track and visualize directional motility in lymphocytes. The generated chemokine gradients are stable for over 24 hours and the viability of the cells is amazingly good, allowing for measurements of chemotaxis over prolonged time frames.

I am also using the ibidi Gas Incubation and Temperature Control Systems, which are robust and guarantee perfect stability. Very importantly, the product developers at ibidi provide a great support for the implementation of their systems. Thanks to ibidi for developing cell imaging products of such great quality!"

Loïc Dupré PhD
INSERM UMR 1043
Purpan University Hospital
Toulouse
France

http://www.cptp.inserm.fr/


PD Dr. Peter J. Hanley, University of Münster, Münster, Germany

"In the past 5 years, we have extensively used the ibidi µ-Slide Chemotaxis 2D to perform time-lapse images of primary mouse macrophages migrating in a chemoattractant gradient. We are most grateful that this device was developed, otherwise it would have been technically difficult to perform robust chemotaxis assays over long time periods (in our case, up to 14 h). By adding a little food coloring dye to the chemoattractant solution one can observe that the dye requires days to equilibrate with the opposing reservoir. Gradient kinetics can be measured by substituting the chemoattractant for a fluorescent dye (with similar molecular weight) and taking serial confocal fluorescence images in the observation area, the narrow channel which connects the two reservoirs.“

PD Dr. Peter Hanley
University of Münster
Institute of Molecular Cell Biology
Münster
Germany

www.uni-muenster.de/Biologie.AllgmZoo/

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