• µ-Slide VI<sup> 0.4</sup>
  • µ-Slide VI<sup> 0.4</sup>

µ-Slide VI 0.4

A 6 channel μ-Slide suitable for multiple immunofluorescence stainings under both static and flow conditions

  • All-in-one chamber that simplifies immunofluorescence protocols
  • Homogeneous cell distribution over the channel surface, regardless of handling practices
  • Cost-effective experiments with small numbers of cells and low volumes of reagents

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Cat. No. Description Pcs./Box
80606 µ-Slide VI 0.4, ibiTreat, tissue culture treated, sterile 15
80602 µ-Slide VI 0.4, Collagen IV, sterile 15
80603 µ-Slide VI 0.4, Fibronectin, sterile (available only on request) 15
80604 µ-Slide VI 0.4, Poly-L-Lysine, sterile 15
80605 µ-Slide VI 0.4, Poly-D-Lysine, sterile (available only on request) 15
80601 µ-Slide VI 0.4, hydrophobic, uncoated, sterile 15

Applications:


Technical Features:

  • 30 µl channel volume, which saves on reagent consumption
  • Easy connection to existing tubes and pumps via female Luer adapter
  • Fully compatible with high resolution fluorescence microscopy
  • Also available as a Flow Kit
  • Defined shear stress and shear rate level
 

Specifications:

Adapters Female Luer
Volume per reservoir 60 µl
Number of channels 6
Volume of each channel 30 µl
Height of channels 0.4 mm
Length of channels 17 mm
Width of channels 3.8 mm
Growth area per channel 0.6 cm2
Coating area per channel 1.20 cm2
Bottom: ibidi Standard Bottom  


Define and print your experimental setup


All-in-One-Chamber for Fast Immunofluorescence Protocols


Cell line Madin-Darby canine kidney
(MDCK) cultured in µ-Slide VI 0.4

Blue: nucleus (DAPI)
Green: actin cytoskeleton
(Alexa 488 conjugated antibody)
Red: mitochondria (MitoTracker)
(courtesy ibidi research group)

 

 



The entire observation field is in excellent phase contrast (click here to enlarge)

 
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