• µ-Slide Membrane ibiPore Flow
  • µ-Slide Membrane ibiPore Flow

µ-Slide Membrane ibiPore Flow

A µ-Slide with a porous glass membrane for transmigration and transport studies under both static and flow conditions

  • World's best optical quality with a thin, porous glass membrane
  • Full fluidic access to the apical and basal sides of adherent cells in numerous applications
  • Different pore sizes for various cell types

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Cat. No. Description Pcs./Box
85016 µ-Slide Membrane ibiPore 0.5µm/20% Flow ibiTreat: #1.5 polymer coverslip, tissue culture treated, 0.5 µm porous glass membrane, 20% porosity, sterilized 10
85026 µ-Slide Membrane ibiPore 3.0µm/5% Flow ibiTreat: #1.5 polymer coverslip, tissue culture treated, 3.0 µm porous glass membrane, 5% porosity, sterilized 10
85036 µ-Slide Membrane ibiPore 5.0µm/5% Flow ibiTreat: #1.5 polymer coverslip, tissue culture treated, 5.0 µm porous glass membrane, 5% porosity, sterilized 10
85046 µ-Slide Membrane ibiPore 8.0µm/5% Flow ibiTreat: #1.5 polymer coverslip, tissue culture treated, 8.0 µm porous glass membrane, 5% porosity, sterilized 10
85016-S µ-Slide Membrane ibiPore 0.5µm/20% Flow ibiTreat Trial Pack: #1.5 polymer coverslip, tissue culture treated, 0.5 µm porous glass membrane, 20% porosity, sterilized 2
85026-S µ-Slide Membrane ibiPore 3.0µm/5% Flow ibiTreat Trial Pack: #1.5 polymer coverslip, tissue culture treated, 3.0 µm porous glass membrane, 5% porosity, sterilized 2
85036-S µ-Slide Membrane ibiPore 5.0µm/5% Flow ibiTreat Trial Pack: #1.5 polymer coverslip, tissue culture treated, 5.0 µm porous glass membrane, 5% porosity, sterilized 2
85046-S µ-Slide Membrane ibiPore 8.0µm/5% Flow ibiTreat Trial Pack: #1.5 polymer coverslip, tissue culture treated, 8.0 µm porous glass membrane, 5% porosity, sterilized 2

Applications:

  • Trans-endothelial migration under flow conditions
  • Co-cultivation of cell layers and transport in 2D or in a 3D gel matrix
  • Apical-basal cell polarity assays
  • Skin and lung models with liquid-air interface
  • Cell barrier model assays with apical-basal gradients
  • Cell migration assays based on filters and porous membranes


Time lapse movie of a HL-60 cell (human) that transmigrates in vitro under flow conditions through the porous glass membrane of an ibidi µ-Slide Membrane ibiPore Flow into a 3D collagen matrix with fMLP.

Imaging of a single SiR-Actin stained cell (indicated by pseudo colors) was performed by spinning disc confocal microscopy (20x objective) with a time interval of 30 s.
(Scale bar = 10 um; arrow indicates direction of flow at a shear stress of 1 dyne/cm2; pore size = 3 um; Courtesy R. Pick, Munich, Germany)

Technical Features:

  • SiMPore’s G-FLAT™ Microporous Glass Membranes
  • Cross-channel structure with a porous optical membrane in between
  • Excellent optical properties, comparable to a coverslip
  • Pore sizes 0.5 µm, 3 µm, 5 µm, or 8 µm
  • Membrane thickness 0.3 µm (300 nm) 
  • For use with objective lenses with a working distance >0.5 mm 
  • Fully compatible with the ibidi Pump System 
  • Defined shear stress and shear rate levels
    (For details see Application Note 11)


 

Specifications µ-Slide:

Total coating area 4.50 cm²
Bottom:  ibidi Polymer Coverslip
Main Channel (Lower Channel):
Adapters Female Luer 
Volume 50 µl
Height 0.4 mm
Length 25 mm
Width 5 mm
Growth area 1.25 cm²
   
Upper Channel:
Adapters ibidi Adapter for 
20/200 µl pipet tips
Volume 55 µl
Height various
Height over membrane  1.3 mm
 


Specifications ibiPore Membrane:

Material  SiO2 (glass)
Thickness 0.3 µm (300 nm)
Membrane size 2 mm x 2 mm
Porous area 1.77 mm x 1.84 mm
Restrictions for objective lenses Working distance >0.5 mm
Maximum pressure accross the membrane 100 mbar
Pore layout Hexagonal spacing
Available variations

Pore Size
0.5 µm
3 µm
5 µm
8 µm

Porosity
20%
5%
5%
5%

Pore-to-pore distance
1 µm
12 µm
20 µm
32 µm

Endothelial cells on 3 µm ibiPore membrane. Immunofluorescence overlay image of phase contrast, DAPI (blue), VE-Cadherins (green), F-Actin (red).

 

Fibroblast cells on 3 µm ibiPore membrane.
Phase contrast image, objective lens 4x.

Recommendations for Applications: Pore Size & Porosity

Type of Membrane Applications Example Cell Types
0.5 µm pores,
high porosity (20%)
Permeability and transport studies,
co-culture models.
With this model, cell migration is not possible.
Lung cells, epithelial cells
3.0 µm pores,
low porosity (5%)
Transendothelial migration Leukocytes (neutrophils, T-cells)
5.0 µm pores,
low porosity (5%)
Invasion, migration Monocytes, macrophages, lymphocytes
8.0 µm pores,
low porosity (5%)
Invasion, migration Tumor and cancer cells, endothelial and epithelial cells, fibroblasts, osteoblasts, melanoma, glioma

The bigger the cell – the bigger the recommended pore size.


Recommended Pore Sizes for Different Applications


Cross Section of the
µ-Slide Membrane ibiPore Flow


 

Composition of the
µ-Slide Membrane ibiPore Flow


3D View with Cross-Channel Structure
and Central ibiPore Membrane

Application Examples

Endothelial Barrier
Cultivation of a cell monolayer on one side of the membrane.

 

Trans-Endothelial Migration in 2D
Cultivation of a cell monolayer on one side of the membrane. Under flow conditions, the rolling, adhesion, and transmigration of suspended leukocytes can be observed.

 

Cell Transport in a 3D Gel Matrix
Under flow conditions, the rolling, adhesion, and transmigration of leukocytes towards cancer cells in a 3D matrix, producing chemoattractants, can be observed.

Co-Culture and Cell Barrier Assay
Cultivation of separate cell monolayers on each side of the membrane. Signaling, co-culture, and transport studies are all possible.

 

Apical-Basal Cell Polarity Assays
Chemical factors inside of a 3D gel matrix lead to the polarization of a cell monolayer, which is cultured on the other side of the membrane.

 

Skin and Lung Models with Air-Liquid Interface
Polarized cell monolayer on one side of the membrane exposed to airflow (e.g., lung epithelial cells, or skin models).


SEM Images of the Porous Glass Membrane

3 µm pores


3 µm single pore

0.5 µm pores

0.5 µm single pores


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