µ-Slide III³ⁱⁿ¹

A μ-Slide for switchable chemotatic gradients that merges 3 separated liquids into one channel

Dynamic fluidic gradients with a maximum time resolution
Alternating gradient fields for chemotactic cell trapping

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Cat. No.	Description	Pcs./Box
80316	µ-Slide III 3in1, ibiTreat, tissue culture treated, sterile	15
80312	µ-Slide III 3in1, Collagen IV, sterile	15
80313	µ-Slide III 3in1, Fibronectin, sterile (available only on request)	15
80314	µ-Slide III 3in1, Poly-L-Lysine, sterile	15
80315	µ-Slide III 3in1, Poly-D-Lysine, sterile (available only on request)	15
80311	µ-Slide III 3in1, hydrophobic, uncoated, sterile	15

Applications:

Fluidic assays with up to three different liquids
Cell sorting with laser traps
Fluidic focusing of inner lane

Technical Features:

3 channels ending in 1
Microfluidic bi-directional gradient generator
Fully compatible with the ibidi Pump System and other pumps
The flow is always laminar
Contains a millimeter scale bar
Also available as a Flow Kit

Specifications:

Adapters	Female Luer
Volume per reservoir	60 µl
Number of channels	3 in 1
Total channel volume	60 µl
Height of all channels	0.4 mm
Width of channels thin/thick	1/3 mm
Total growth area	1.23 cm²
Coating area	3.05 cm²
Distance of scale bars	1 mm
Bottom: ibidi Standard Bottom

Flow Setup for Switchable Chemotactic Gradients

Gradient steepness at different positions. The flow is generated by the ibidi Pump System.

Overlapping gradients for chemotactical cell trapping. Switching times of some seconds allow fast gradient switching, so that cytoskeletal dynamics can be observed using fluorescence microscopy.