µ-Slide Chemotaxis ^2D

Used to investigate chemotaxis of slow migrating adherent cells on 2D surfaces, e.g., cancer cells, endothelial cells and fibroblasts

Chemotaxis measurement in real-time
Stable gradients for long-term experiments
Reproducible results with reliable and user-independent data

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Cat. No.	Description	Pcs./Box
80306	µ-Slide Chemotaxis 2D, ibiTreat, tissue culture treated, sterile	10
80302	µ-Slide Chemotaxis 2D, Collagen IV, sterile	10

Applications:

Chemotaxis of cancer cells, endothelial cells and fibroblasts
Chemotaxis of other slow migrating adherent cells
Migration assays with simultaneous cell tracking using microscopy

Technical Features:

3 chambers on one slide for parallel assays
Ready to use, i.e. no assembly required
Linear gradients, which remain stable for over 48 hours
Made for high-end video microscopy, including fluorescence microscopy

Specifications:

Chemotaxis chambers on slide	3
Volume per chamber	80 µl
Observation area	2x1 mm²
Coating area per chamber - When coating full chamber - When coating observation area only	2.40 cm² 0.39 cm²
Distance between chambers	21.5 mm
Total height with plugs	12 mm
Volume chemoattractant	18 µl
Bottom: ibidi Standard Bottom

Define and print your experimental setup

Application Examples


Observation area is recorded with a 4 x objective.	Chemotaxis of HT-1080 cells, which are migrating towards FCS, using low resolution microscopy for a maximum of migration data	High resolution microscopy during chemotaxis and cell polarization, using the HT-1080 LifeAct cell line

Necessary Equipment

Basic requirements:

Inverted microscope with a 5x objective and 10x phase contrast
Basic camera for time lapse movies
Heated microscopy system (required for experimentation on most mammalian cell types)

Recommended extensions:

Motorized stage for parallel data acquisition
Auto focus
CO₂ Incubation system

Data Analysis

1. Chemotaxis and Migration Tool

To facilitate analysis of results from chemotaxis experiments (time stacks) ibidi has developed this software analysis tool. The tool provides various types of graphs and statistical tests for advanced analysis of experimental data.

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2. Automated Quantitative Chemotaxis Image Analysis - WimTaxis

Manual acquisition and evaluation of 2D cell migration is time consuming and lacks in objectivity. Wimasis, the certified partner of ibidi for quantitative image analysis, developed an analysis solution to quantitatively evaluate 2D chemotaxis and migration. The Image Analysis solution WimTaxis generates fast results, without the need for extra hardware or software.

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