Chemotaxis and Migration Tool
A free software tool for data analysis from time stack chemotaxis experiments, based on the National Institute of Health’s (NIH) ImageJ image processing system
- A free and platform independent solution
- Provides various graphs and statistical tests that allow advanced analysis of chemotaxis experiments
Download the Chemotaxis and Migration Tool for free here.
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Instructions Chemotaxis and Migration Tool Version 2.0 (english) - stand alone
Instructions Chemotaxis and Migration Tool Version 1.01 (english) - ImageJ Plugin
Instructions Chemotaxis and Migration Tool Version 1.01 (deutsch) - ImageJ Plugin
The Chemotaxis and Migration Tool is an ImageJ plug in. It is freely available and platform independent which means that the tool can be run on all major operating systems. The tool provides various graphs and statistical tests that allow advanced analysis of experimental data.
After cell tracking, the cells‘ paths can be plotted and analyzed for chemotactical effects. The simple to use microscopic calibration system provides access to a variety of parameters. For specific analyses, sector tools are available that count cells in angular or circular areas, known as Rose plots. A set of statistical tests complete the software package.
All calculations can be immediately visualized using the provided histograms and other diagrams. Optionally, all data can be exported for further analyses. With a complete set of migration data, the user is able to quantify chemotaxis and random migration.
The Chemotaxis and Migration Tool, together with the µ-Slide Chemotaxis 2D or the µ-Slide Chemotaxis 3D enables users to analyze in minute detail the effect of drugs on the chemotactic behavior of cells.
For tracking cells in a time stack, ibidi recommends using the Manual Tracking plug in available from: http://rsb.info.nih.gov/ij/plugins/track/track.html