Cytotoxicity Image Analysis - WimCytotoxicity

A web-based quantitative image analysis of cytotoxicity assays with automated detection of live and affected/dead cells

Complete solution for cytotoxicity assays - only a few steps from sample preparation to image analysis
Objective and reproducible analysis
Easy and fast data processing - results within hours
No more manual cell counting

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Cat. No.	Description	Pcs./Box
30007-500	WimCytotoxicity Quantitative Cytotoxicity Image Analysis, 500 images	500
30007-1000	WimCytotoxicity Quantitative Cytotoxicity Image Analysis, 1,000 images	1000
30007-2000	WimCytotoxicity Quantitative Cytotoxicity Image Analysis, 2,000 images	2000
30007-5000	WimCytotoxicity Quantitative Cytotoxicity Image Analysis, 5,000 images	5000
30007-10000	WimCytotoxicity Quantitative Cytotoxicity Image Analysis, 10,000 images	10000
30007-20000	WimCytotoxicity Quantitative Cytotoxicity Image Analysis, 20,000 images	20000
30007-50000	WimCytotoxicity Quantitative Cytotoxicity Image Analysis, 50,000 images	50000
30007-100000	WimCytotoxicity Quantitative Cytotoxicity Image Analysis, 100,000 images	100000

FAQ

FAQ Image Analysis

Applications:

Quantification of cytotoxicity assays
Quantification of live-dead stainings
Image analysis and data interpretation of vitality tests

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The use of fluorescent dyes to assess cellular viability under the effect of cytotoxic agents is an extended practice in pharmaceutical research. It is a key assay in screening the cytotoxic potential of new drug compounds, as well as the study of cell-mediated cytotoxicity, or the evaluation of the effects of cytotoxic pollutants in cells.

Wimasis, the certified partner of ibidi for quantitative image analysis, developed an analysis solution to quantitatively evaluate viability stainings in cell culture. The Image Analysis solution generates fast results, without the need for extra hardware or software.

First, perform your cytotoxicity experiments, with a subsequent fluorescence staining. Then, simply upload your data and receive a summary of results, via E-mail, and download the detailed results.

Analysis Data Contains:

Total cell count: number of all visible cells in the culture
Number of affected/dead cells stained by the cytotoxicity fluorescence marker
Viability of cells (percentage of affected/dead cells of the whole population)

Because every experimental setup is unique, the WimCytotoxicity tool is engineered with the flexibility to adapt to the needs of every researcher. If your cytotoxicity assay does not fit the requirements above, send us a quick note, or reach us at info@ibidi.de, so that we can find a solution that fits your experiment.

Detailed Description of the Analysis:

For the input data, WimCytotoxicity uses fluorescence microscopy images with two different dyes: one dye for the viable cell population and another one for the cytotoxicity-affected cells. Optionally, an extra nuclear dye can be applied to stain cellular nuclei, which will then improve the accuracy of the cell detection algorithm.
The quantification is based on the detection of the whole cell population and the identification of the cells affected by the cytotoxic agents.